Flashcards in Lab - Determination of Nitrogen/Crude Protein Deck (14)
What method was used in the experiment?
To be more specific, the ____ modification of the Kjeldahl technique was used
three phases of the Winkler modification of the Kjeldahl technique
digestion with acid
distillation to release the ammonia
titration with standard sulfuric acid
digestion phase involves decomposition of organic nitrogen compounds c/o of which acid?
in acid digestion phase, organic C and H become
CO2 and H2O
in acid digestion phase, H2SO4 reduced into SO2. SO2 reduces nitrogenous material into __
in acid digestion, reduction in time is done y adding K2SO4 or Na2SO4. Why?
they may raise the boiling point of the mixture
in the distillation stage to release ammonia, what acid is used?
protein is assumed to have __% of Nitrogen
What indicator was used in the experiment?
B. methylene red
What distillation apparatus was used?
Markham distillation apparatus
Methods for determining nitrogen & crude protein are directly based on
A. Speed of result delivery
B. Presence of Functional groups in the molecule
C. Both A & B
The ultimate reference method for determining the concentration of protein is the ___
Analysis of Nitrogen content