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Flashcards in Lab Diagnosis Deck (64):
1

Viral shedding is greatest when?

During Early stages of infection

2

Viral culture results can decrease rapidly inhow many days

3

3

These can be added to prevent tissues from dryinh

Transport media
Saline
Trypticase soy broth

4

Buffered transport media contain proteins go protect less stable viruses

Albumin
Gelatin
Serum

5

Thepresence of this will disrupt the host cell and results to loss of viral viability

Ice crystals

6

What spx can be used to isolate eneteroviruses after the cessation of respiratory shedding

Stool

7

Major causes of aseptic meningitis

Enteroviruses

8

4 major methods in to diagnose viral infections

Direct detection
Nucleic acid based detection
Cell cultures
Serologic assays

9

Not as sensitive as cell culture but can offer quick results for rapid therapy

Direct detection

10

Bright field microscopy can be used to what virus

Poxvirus

11

Type of microscopy that is useful to detect non culturable virus

Electron microscope

12

It is the distinctive and characteristic visual changes in infected cells

Cytopathic effects

13

CPE can be detected in _____ using bright field

Cell scrapings

14

Smear that detect cowdry type A bodies

Tzanck smear

15

Owl eyes is seen in what virus

HSV AND VZV

16

_________ smear reveal HPV

Papanicolaou

17

Squamous cells with enlarged nucleus and non staining halo (perinuclear halo)

Koilocytes

18

Eosinophilic cytoplasmic inclusions in neurons

Negro bodies

19

Negro bodies are seen in

Rabies

20

EIA using multiwell microtiter plates can detect what virus in
Respiratory spx
Serum or plasma
Stool
Cutaneous lesions and conjuctival

RSV and influenza A
Hep b virus and HIV -1
Enteric adenovirus
HSV

21

EIA in single test platforms detects these viruses in
Respiratory spx
Rectal swabs
Serum

RSV, influenzavirus A and B
Rotavirus and enteric adenovirus
West nile fever virus

22

EIA negative results are confirmed with

Cell culture or IF or nucleic acid based test

23


This test gives a quantitative result

Nucleic acid based detection

24

Combination of flow cytometry and PCR for mutiplex detectio

luminex system

25

Nucleic acid based assay that primarily detects blood pathogens

Gene amplification technique

26

Rapid subtyping for influenza A virus

Microarray assay

27

Luminex system detects how many type or subtype pathogens and wihtin how many hrs

20
5 hrs

28

The gold standard

Viral isolation

29

Three methods for viral isolation

Cell culture
Animal inoculation
Embryonated eggs

30

Most commonly used isolation method

Cell culture

31

Coxsackie virus require what animal for isolation

Suckling mice

32

Isolation of Influenza virus is enhanced in

Embryonated eggs

33

Most of the clinical workload focuses on the detection of

HSV and respiratory viruses

34

Common clinical viruses can be identified within

48 hrs

35

Common clinical viruses

HIPRAE

HSV
Influenza virus A ANDB
PIV 1 - 4
RSV
Adenovirus
Enterovirus

36

Used to indicate culture of cells in vitro

Cell culture

37

Denote the growth of tissues or organ so that the architecture or function is reserved

Tissue culture

38

Cell culture that is obtained from tissue removed from an animal and has minimal cell division

Primary cell culture

39

Tissue in promary cell culture is treated with what enzyme to disperse individual cell further

Trypsin

40

Process of removing cells from the surface, diluting and placing them in a new container

Splitting or passaging

41

Commonly used primary cell culture

Primary monkey kidney cells

42

Can divide but has limited passage ( up to50 gen)

Finite cell cultures

43

Term for having two copies of chromosome

Diploid

44

True or false
As the number of passae increases, cells become more insensitive to viral infection

True

45

Standard finite cell culture

Human neonatal lung

46

Cell culture capable of infinite passage and are heteroploid

Continuous cell cultures

47

HEp2 is derived from

Human laryngeal epithelial carcinoma

48

A549 is dervied from

Human lung carcinoma

49

Vero is derived from

Monkey kidney

50

Developed from combining two cell lines

Mixed cell culture

51

Exhibits rapid growth, plaque formation, And growth ondifferent cell types

HSV

52

HSV produces CPE within

24 hrs

53

Growth on rabbit kidney cells is indicative of what

HSV

54

Produces HSV-like CPE and growns only on diploid fibroblast

CMV

55

Adenovirus CPE appearance

Cluster of grapes

56

Produce classic syncytial formation

RSV

57

Gian multinucleated cells resulting from cell fusion as a consequence of viral infection

Syncytia

58

Cells infected with influenza virus expresses what which binds to RBC

Hemagglutinin protein

59

True or false
(Hemadsorption)
If positive, the rbc will stick or adsorb to the infected cell

True

60

Stain used to detect viral antigen and is used to screen cultures before final negative result

Fluorescent antibody stain

61

Can identify more rapidly vrisuses than xian

Centrifugation-enhances shell vial culture

62

Sa small round flat bottmed tube with screw cap

Shell vial

63

Modifcation of centrifugation-enhanced shell vial culture

Use of flat bottomed microtiter plates

64

Measures the host response rather than the directly detecting the virus

Serologic assays