LEC 14 Flashcards
(52 cards)
def? natural fluorescence found within cells
autofluorescene
def? Any particle which generates signal after passing through the laser beam
event
what is forward scatter proportional to
cell size
what is side scatter proportional to
internal complexity like granularity, vacuoles, or inclusions
T or F: neutrophils will have a higher side scatter than lymphs and monos
T
def? plot that displays a single measurement parameter
histogram
def? plot that display 2 measurement parameters
dot plots
def? ID of cells in a population through the staining and ID of specific markers using monoclonal AB conjugated with fluorochromes
immunophenotyping
def? dye which allows ID of dead cells through a reduction in cell mmb integrity
viability dye
T or F: dead cells can be used in flowcytometry
F
what kind of cells can flowcytometery ID
subgroups
what kind of lymph has granules
large granule lymphs
on a dot plot with SSC vs. FSC: top left will have ____ granules and _____ size
increased, small
on a dot plot with SSC vs. FSC: bottom left will have ____ granules and _____ size
decreased, small
on a dot plot with SSC vs. FSC: top right will have ____ granules and _____ size
increased, big
on a dot plot with SSC vs. FSC: bottom right will have ____ granules and _____ size
decreased, big
what does it mean if a flowcytometer has more lasers
the more lasers the more advanced
routine blood diagnostics and blood count is still done by what today
Coulter counters
def? measuring the physical and chemical properties of cells flowing in a stream of fluid
flow cytometry
t or F: flow cytometry can measure other things besides cells
T
what does flow cytometery use to obtain info about a cell
- forward light scattered
- side light scattered
- fluorescence
what can flow cytometery tell you about leukemias
- morphology
- immunophenotype
- cytogenentics
what component of FC? sheath fluid tank and flow cell
fluidics
what component of FC? lasers and filters
optics
