Lec 20-21

Question 1

Hox genes

Answer 1

Encodes transcription factors, regulate expression of development genes

Question 2

What differences in DNA are used to identify people?

Answer 2

Short tandem repeats: short DNA sequences to repeat varied amount of time in different people

Question 3

What enzymes/molecules need to be added for PCR to replicate the gene of interest?

Answer 3

DNA polymerase, primers, free nucleotides

Question 4

CRISPR/CAS9 in bacteria

Answer 4

1. When virus invades, CAS1/2 protein reads and copies segment of virus’s genetic code
2. CAS1/2 protein inserts copy in CRISPR region of bacteria genome
3. Viral sequences transcribed and attach to CAS9
4. CAS9 searched cell to match sequence. When virus invades CAS9 cuts virus up

Question 5

CRISPR/CAS9 for DNA manipulation

Answer 5

Creates gRNA complementary to sequence thats getting edited, insert gRNA with CAS9 to cell to fix mutation. Can add sequence of DNA to rebuild gene