Lecture 12 - Enzyme Kinetics & Inhibition

Question 0

Maximum number of substrate molecules an enzyme molecule can convert to product per unit time

Answer 0

k_cat (turnover number)

Question 1

What’s the study of the rate at which some processes progresses is called?

Answer 1

Kinetics

Question 2

True or false? A small value of k_cat is better

Answer 2

False, LARGER not smaller.

Question 3

Steps to experimentally determine K_m and V_max

Answer 3

1) Keep E_t constant
2) Use [S1] and measure the product formed (V0)
3) Draw the best straight-line rough the points to estimate V_0
4) Repeat for other concentrations
5) Graph V_0 versus [S]