Lecture 2 Flashcards Preview

Med micro > Lecture 2 > Flashcards

Flashcards in Lecture 2 Deck (34):
1

what are two types of Prokaryotic cells ?

Bacterial
Archaeal

2

Do Prokaryotic cells have internal membranous organelle ?

No

3

Is Translation and Transcription separated in Prokaryotic cells

No
because nuclear envelope is not present

4

Is Bacteria Haploid or Diploid?

Haploid

5

Define Plasmids

double stranded DNA molecules, circular, independent of chromosome
carry genes, sometimes useful bout not needed for basic living

6

Do bacterial cells have sterols?

No
except mycoplasma cells (they still their sterols)

7

What does the cell membrane in eukaryotes contain?

Transport protein for uptake and release of materials
ion pumps to create and maintain membrane potential

8

Define Quorum Sensing

method for communication between bacteria that rely on secretion of and detection of small signaling molecules

9

Where is the cell wall found in Prokaryotes ?

Outside of the cell membrane

10

What is the size of Bacterial Ribosomes?

70s formed os 30s and 50s
30s contain 16s rRNA

11

Where does the Glycolitic , PP and ED pathways occur?

Cytoplasmic Matrix ( between cell membrane and nucleic )

12

Where does the TCA cycle occur?

Cytoplasmic matrix

13

What is Flagella?

Rope-like propellers that extend beyond the cell wall, formed of protein(flagellin)
energy rotation comes from electromotive force

14

What are Spirochates?

helical Gram-negativr bacteria with endoflagella
rotation: flexing and spinning movements

15

Define PILI and FIMBRIAE

thinner that flagella and also extend beyond the cell wall
formed of protein PILIN used for attachment

16

How prokaryotic cells transport molecules?

they use transmembrane channels for large molecules to cross their membranes

17

Why Gram Negative bacteria have a problem when it comes to transporting molecules?

They possess outer membrane that prevents them from transportation since the protein already have folded in periplasmic space

18

What are the protein in Prokaryotes that are homologous to Actin and Tubulin in Eukaryotes?

Tubulin homolog ---binds and hydrolyzes GTP
important in bacterial cell division

Actin homolog ---affects cel shape Bacilus subtitles

19

What is the location of a cell wall in Prokaryotes?

just outside of the cell membrane

20

Bacterial cell wall contain Peptidoglycan

True/False

True

21

Archaeal Cell walls lack Peptidoglycan

True/ False

True
but they still stain gram position and negative

22

Define VOLUTIN

storage form of polyphosphate

23

Define Glycocolax

Capsules and slim layers outside of the cell wall
not need for growth
important for survival in a host organism

24

Define Biofilms

organized layers of microbe cells associated with surfaces

25

What is the function of Peptidoglycan?

Provides the structure and shape and protects the cell from osmotic forces
assists some cells in to other cells
unique to bacteria

26

What two carriers are needed for Synthesis of Peptidoglycan?

UDP
Bactoprenol

27

Effect of Penicilin use during Peptidoglycan synthesis

inhibition of the transpeptidation reaction

28

Effect of Bacitracin use during Peptidoglycan synthesis

blockage of dephosphorilation of bactophenol pyrophosphate

29

Effect of Cycloserine use during Peptidoglycan synthesis

blockage of conversion of L alanine to D alanine

30

Effect of Vancomycin use during Peptidoglycan synthesis

inhibits transpeptidation reaction only for the bacteria having D-alanine D-alanine terminal dipeptide

31

Why Gram Positive Bacteria stains purple ?

because the thick peptidoglycan retains crystal violet dye

32

can you use Gram staining for Acid-fast bacteria?

no because their cell wall also contains waxy lipids
it would stain both

33

What is Lipopolysacharide used for ?

it is used to classify bacteria

34

gram positive vs Gram negative basal bodies

Gram negative 4
Gram positive 2