LIVER FUNCTION TEST part 4

Question 1

uses Diazotized sulfanilic acid for Bilirubin used in serum

Answer 1

Van Den Berg Reaction

Question 2

Van Den Berg Reaction uses this acid in serum.

Answer 2

Diazotized sulfanilic acid

Question 3

the diazotized sulfanilic acid is formed by reacting sulfanilic acid with _________________

Answer 3

sodium nitrate and hydrochloric acid

Question 4

This is often subjective and the test involves diluting serum with saline until it visually
matches the color of a 0.01% potassium dichromate solution

Answer 4

Icterus Index

Question 5

Icterus Index test involves diluting serum with __________ until it visually matches the color of a ____________________

Answer 5

saline
0.01% potassium dichromate solution

Question 6

What are the interferences in Icterus Index?

Answer 6

 Carotene
 Xanthophyll
 Hemoglobin

Question 7

In DIRECT SPECTROPHOTOMETRIC METHOD, what is the wavelength at which bilirubin in serum is measured?

Answer 7

455nm

Question 8

the absorbance of hemoglobin is 455 nm which can be corrected by subtracting the absorbance at ____________

Answer 8

575nm

Question 9

Problem causing jaundice occurs before liver metabolism

Answer 9

Pre-hepatic Jaundice

Question 10

What caused the pre-hepatic Jaundice?

Answer 10

Increased amount of bilirubin being presented in the liver

Question 11

Unconjugated hyperbilirubinemia

Answer 11

PRE-HEPATIC JAUNDICE

Question 12

Intrinsic liver disease

Answer 12

HEPATIC JAUNDICE

Question 13

In Gilbert’s Syndrome, this is reduced in the expression of gene.

Answer 13

UGT1A1

Question 14

 intermittent hyperbilirubinemia
 inherited bilirubin derangement
 Reduced conjugating capability of the liver

Answer 14

Gilbert’s Syndrome

Question 15

 has a defective hepatic uptake of bilirubin

Answer 15

Gilbert’s Syndrome

Question 16

has a defective conjugation of bilirubin due to the deficiency of the enzyme UDPGT

Answer 16

Crigler-Najjar Syndrome

Question 17

• Chronic unconjugated hyperbilirubinemia
• Molecular defect w/in the gene involved with bilirubin conjugation
• Rare & more serious disorde

Answer 17

Crigler-Najjar Syndrome

Question 18

 total UDPGT deficiency
 complete absence of enzymatic bilirubin conjugation

Answer 18

Type I Crigler-Najjar Syndrome

Question 19

 partial UDPGT deficiency
 the mutation causing a severe deficiency of the enzyme for Conjugation

Answer 19

Type II Crigler-Najjar Syndrome

Question 20

defective hepatic excretion of bilirubin

Answer 20

Dubin-Johnson Syndrome

Question 21

What caused the Dubin-Johnson Syndrome?

Answer 21

deficiency of the canalicular transporter protein (MDR2/CMOAT)

Question 22

defective hepatic excretion of bilirubin; has normal gall bladder function

Answer 22

Rotor Syndrome

Question 23

What causes the Rotor Syndrome?

Answer 23

reduction in the concentration or activity of intracellular binding proteins.

Question 24

TRUE OR FALSE:
Liver biopsy does NOT show dark pigmented granules

Answer 24

TRUE

Question 25

it has a presence of circulating antibody against UDPGT

Answer 25

Lucey-Driscoll Syndrome

Question 26

 deposition of bilirubin in the nuclei of brain & nerve  cells-cell damage & death of newborn

Answer 26

Kernicterus

Question 27

 Usually results from biliary obstructive disease (e.g. gallstones, tumors)  Prevents the flow of conjugated bilirubin into the bile canaliculi  Clay-colored stool

Answer 27

POST-HEPATIC JAUNDICE

Question 28

What happens when bilirubin is exposed to light?

Answer 28

Photo-oxidize

Question 29

conjugated bilirubin that is covalently bound to albumin

Answer 29

Delta Bilirubin

Question 30

the ______________ enhances or accelerates the unconjugated bilirubin reaction with diazotized sulfanilic acid

Answer 30

accelerator agents