MFD SBA

Question 1

Species highly correlated with BANA test -

Answer 1

P. Gingivalis

other red complex too

Question 2

Constituent of MacConkey inhibits growth of gram positive bacteria -

Answer 2

bile salts

Question 3

Synder test yellow result; gram positive rod

Answer 3

lactobacillus

Question 4

pathogenicity of Staphylococcus aureus -

Answer 4

coagulase, hyaluronidase, deoxyribonuclease, exotoxins, superantigens; beta lactamases

Question 5

Dental hand piece decontamination

Answer 5

autoclave

Question 6

Which bacterial component causes an acute inflammatory reaction

Answer 6

LPS

Question 7

Which bacteria is beta haemolytic on blood agar

Answer 7

S. pyogenes, P. gingivalis,

S. aureus

Question 8

Which bacteria is alpha haemolytic on blood agar

Answer 8

oral streptococci

Question 9

Which bacteria is gamma haemolytic on blood aga

Answer 9

staph

s. epidermidis

Question 10

What factors contrinute to the pathogenicity of aggregatibacter actinomuycetemomitans:

Answer 10

Adhesins, bacteriocins, invasins

Trypsin like Proteases

Surface antigens

Heat shock proteins

Antimicrobial resistance

Localised aggressive PD

Leukotoxins which kill monocytes and neutrophils

Question 11

Which bacteria is a spirochete

Answer 11

T. Denticola

Question 12

What mechanism moves drugs from the blood to tissues?

Answer 12

distribution

Question 13

MSB agar is to isolate which bacteria?

Answer 13

contains bacitracin which allows strep mutant and sobrinus to grow

Question 14

Aetiological factor for carie

Answer 14

S. mutans

Question 15

Infection control

Answer 15

cross infection control, hand washing, waste disposal (separate clinical and domestic), vaccination, medical history

Question 16

What is the route of transmission for HBV?

Answer 16

Blood

Question 17

Name a spore forming bacteria?

Answer 17

Clostridium difficile
c tetani
c botulinum

Question 18

What is the definition of a microbiome?

Answer 18

total Mo in a particular environment

Question 19

What protein structure is a factor for pathogenicity for C.tetani?

Answer 19

Neurotoxin

Question 20

What protein structure is a factor for pathogenicity for N. gonorrhoea?

Answer 20

OP surface protein

Question 21

What determines critical pH of saliva?

Answer 21

Peoples saliva composition varies so they therefore have different buffering capacitities

Question 22

Name the gram+ rod in synder in dental plaque

Answer 22

Lactobacillus

Question 23

What polysaccharide is formed by strep and what mutans required a primer?

Answer 23

Fructans and glucans

glucans need primer except -
primer indépendant GTF which was called GTFSi ( comprise of )α-1,3 and α-1,6 bonds E.g. the GTF of S. gordonii

Question 24

1)What salivary component inhibits the growth of bacteria by binding to enzyme co-factor?

Answer 24

Lactoferrin

Question 25

How is drug resistance developed between bacteria?

Answer 25

Conjugation

Question 26

Mechanisms that cause penicillin resistance in bacteria?

Answer 26

Beta lactamase production

Question 27

In muscarinic stimulation PIP activates what membrane bound protein causing moderate amount of protein secretion?

Answer 27

DAG

Question 28

1)What salivary factor determines critical pH?

Answer 28

Ca2+ and PO4 concentration.

Question 29

What component of the secretory cell membrane lowers Na in the cell?

Answer 29

Na/K ATPase

Question 30

What contributes to the pathogenicity of s. aureus?

Answer 30

Exo/enterotoxin

Question 31

Why is CLED used for urine?

Answer 31

Electrolyte deficiency prevents swarming of proteus. Yellow = E-coli, Blue = Proteus.

Question 32

2)What component of MaConkey agar is inhibitory to gram -ve bacteria?

Answer 32

Sodium deoxycholate, bile salts . Selects for gram –ve bacteria.

Question 33

What is a product of tryptophan metabolism?

Answer 33

Skatole

Question 34

2) What bacteria would be present in a positive BANA test?

Answer 34

P. gingivalis, Treponema denticola, Tannerella forsythia

Question 35

What factor contributes to the pathogenicity of actinomyces actinomycetemcomitans?

Answer 35

aggressive periodontitis - the bacteria secretes leukotoxins which kill monocytes and neutrophils. Adhesins/invasins, proteases, antimicrobial resistance

Question 36

What drug stimulates the release of insulin?

Answer 36

Sulphonylureas (e.g. glibenclamide

Question 37

What type of reaction changes the ketone group to a hydroxyl group on warfarin?

Answer 37

Phase 1 reaction.

Question 38

What bacteria is alpha haemolytic on blood agar?

Answer 38

Oral streptococci

Question 39

What bacteria is gamma haemolytic on blood agar?

Answer 39

Staphylococcus species (s. epidermidis)

Question 40

A deficiency in what causes angular stomatitis?

Answer 40

Riboflavin

Question 41

What would you clean a blood spillage with?

Answer 41

Hypochlorite

Question 42

What causes black colonies on agar?

Answer 42

- Blood agar- PORPHYROMAS GINGIVALIS – porphyrin pigments contain haem

Question 43

What is the main etiological factor in dental caries?

Answer 43

Oral bacteria associated with caries (S. mutans) use ATPase to pump H+ out of cells

Question 44

Which of these stabilizes calcium and phosphate?

Answer 44

Proline-rich proteins (PRPs) (statherin also does along with PRPs)

Question 45

Which of these is a virulence factor of S.mutans when sugar is low?

Answer 45

no clue tbh Carbohydrate utilisation( Saccharolytic)? good at converting lots of different sugars to acids? Expopolysaccarides

Question 46

What sweetener would you find in your drink?

Answer 46

Stevia

Question 47

Which of these is a pathogenic factor of N. gonorrhoea

Answer 47

Pilli - Genetic exchange (conjugation)

Question 48

After adding calcium hydroxyapatite to water at pH 7 and forming an equilibrium which is true?

Answer 48

hydroxyapatite will dissolve until an equilibrium is reached

Question 49

Which of these factors is a pathogenicity factor for S.mutans in acidic environment?

Answer 49

* Reduced permeability of cell membrane to H+. * Induction of H+-translocating ATPase (expels protons from cells). * Induction of alkali production systems (arginine deiminase or urease). * Induction of stress proteins that protect enzymes and nucleic acids from denaturation

Question 50

Which of these is true about LPS?

Answer 50

Some things in my notes about LPS • Endotoxin • Found only in Gram-negative bacteria • Present in the outer layer of the outer membrane • Released during growth or when bacteria die and lyse • Causes fever, septic shock and local inflammation

Question 51

What is Chlorhexadine?

Answer 51

* Bisbiguanide (cationic – attracted to –ve charge) * Disrupts cell membrane phospholipids * High substantivity* (up to 12 hours)- relates to the persistence of its antimicrobial activity of the surface.

Question 52

What is a product that causes halitosis post hydrolysis?

Answer 52

Amino acids

Question 53

Which of these is not killed buy autoclave?

Answer 53

Prions