Michaelis Menton

Question 1

Michaelis menton

Answer 1

v = Vmax S / Km + S

Question 2

Km and how this affects michaelis menton

Answer 2

Km lower than S, then Vmax is reached

Km higher than S, linearly dependent on S (no S at bottom of equation)

Question 3

Rate units of association and dissociation constant

Answer 3

M-1

M

Question 4

K’m

Answer 4

Km x (1+[I] / Ki)

Question 5

Competitive inhibitors

Answer 5

Changes Vmax and Km
v = Vmax S
K’m + S

Question 6

Non competitive inhibitors

Answer 6

v = Vmax’ S
Km + S

Where Vmax’ is Vmax/Km’

Question 7

Burst amplitude

Answer 7

E X (k’1 / k1 + k2)2

Question 8

Burst rate constant

Answer 8

k’1 + k2

k’1 is k1 [S]

Question 9

kobs from a Fl graph

Answer 9

kobs = ln2/ t1/2

For first order this is just log

Question 10

One step ligand binding

Plot

Answer 10

kobs = L X k+1 + k-1 (if not reversible this is only dependent on L)

Slope k+1 intercept k-1

Question 11

Two step ligand binding
Fast slow
Slow fast
Plot

Answer 11

kobs = K1k2L/K1+L (+k-2)

kobs = k+1 + k-1 x (1 + L/K1)

Intercept k-2
Half Vmax = 1/K1

Question 12

Mass spec equations

Answer 12

m/z = MW + NH+
n

For the second peak (smaller) all the n’s are plus 1
n = Small -1 / big - small

Question 13

Time of flight equation

Answer 13

m/z = 2Vt2 / L2

T = m/2zv ^ 1/2 X d

Question 14

Quantum yield

Answer 14

Excited that fluorescence

Total number of excited

Question 15

Energy transfer efficiency

Answer 15

Ro6 / Ro6 + R6

Question 16

Molar ellipicity and mean residue molar

Near and far UV

Answer 16

L-R or change A/c L

Change A/c L n

190-250 250-300

Question 17

How to find conformational stability

How many bonds to break

Answer 17

Calculate K = FU/FF from the transition region
Then convert to G = -RTlnKeq
Plot G against denaturation to get y incept as stability
How many bonds to break? Divide by hydrogen bond which is 8-20 kJ
(About 5 to break protein)

Question 18

Fractional saturation

Answer 18

EL/E total, can be found by rearranging to get L/L + Kd

Question 19

Multiple identical sites

Answer 19

Kd = E L /EL X EL L/ EL2
= K1 X K2 = E L2 /EL2
Fractional sat becomes nL/L +Kd

Question 20

Scatchard plot

Answer 20

Frac/ L against frac
Slope 1/Kd
Intercept on X is n

Question 21

Hill plot

Answer 21

Log Frac/n-frac against log L

Question 22

Multiple non identical independent sites

Answer 22

Frac = n1 L /L + K1 X. n2 L /L + K2

Question 23

Hill analysis

Answer 23

Plot of log Frac/1-Frac) against logL

Slope is n intercept Kd

Question 24

Mathews coefficient

Answer 24

Vm = Volume cell /MW Z X

Question 25

Atom abundance

Nerst equation

Answer 25

H 99.9
P 100
C 1.07
N 0.37

E PDI = EGSH - (RT/nF) ln Keq

Question 26

Correlation time

Hetero nuclear decoupling

Answer 26

Tm = 4pi r3 n
3kb T

Hd u 3cos2 theta -1