Micro Flashcards by yumi Kojima

Name the flaviviridae viruses

Hep C

West nile, Dengue, Zika, Yellow fever, St Louis & Japanese encephalitis

How well did you know this?

Not at all

Perfectly

Name cell line used to grow Enterovirus

Primary Monkey Kidney

How well did you know this?

Not at all

Perfectly

Name the season for Enterovirus

Summer Fall

How well did you know this?

Not at all

Perfectly

Give example of Enteroviruses

SS RNA

Polio

Coxsackie A

Coxsackie B - Myocarditis

Enterovirus

Echovirus

Rhinovirus

How well did you know this?

Not at all

Perfectly

Name the season for Rota virus

Winter

How well did you know this?

Not at all

Perfectly

Name 2 Non enveloped ssDNA virus

Parvoviridae - Parvovirus B19

Bocavirus

How well did you know this?

Not at all

Perfectly

Name 3 nonenveloped dsDNA virus

Adenoviridae

Papillomaviridae - HPV

Polyomaviridae - JC & BK virus

How well did you know this?

Not at all

Perfectly

Name 3 Enveloped dsDNA virus

Herpesviridae

Hepadnaviridae -HBV

Poxviridae - Smallpox, Molluscum

How well did you know this?

Not at all

Perfectly

Name 3 nonenveloped ssRNA virus

Picornaviridae - Enterovirus, Hep A, Rhinovirus

Calciviridae- Norovirus

Hepeviridae - Hep E

How well did you know this?

Not at all

Perfectly

Name 10 enveloped ssRNA virus

Flaviviridae - HCV, Yello fever, Dengue, WNV, St. Lous/japane enecephalits

Togaviridae - Rubella EEE, WEE

Retroviridae - HIV, HTLV

Orthomyxoviridae - Influenza

Paramyxoviridae - RSV, Parainfluenza, Mumps, Measles

Rhabdoviridae

Coronaviridae

Arenaviridae

Bunyaviridae - Hatavirus

Delta virus - Hep D

How well did you know this?

Not at all

Perfectly

Name 1 nonenveloped dsRNA virus

Reoviridae- rotavirus

How well did you know this?

Not at all

Perfectly

Name the cell lines used to grow the following

Enterovirus

Rhinovirus

Influenza, Mumps, parainfluenza

Adenovirus

RSV

CMV

HSV 1 &2

Enterovirus - PMK

Rhinovirus - HDF

Influenza, Mumps, parainfluenza - PMK

Adenovirus - Hep2/HeLa

RSV - - Hep2/HeLa

CMV- HDF

HSV 1 &2- HDF

How well did you know this?

Not at all

Perfectly

Duncan disease is associated with?

EBV

X-linked lymphoproliferative disease - SH2D1A (SAP) gene

overactive immune response to EBV, resulting in fulminant infectious mononucleosis, B cell lymphoma, aplastic anemia and dysgammaglobulinemia

How well did you know this?

Not at all

Perfectly

Ramsay Hunt Syndrome

VZV

Reactivation in the geniculate ganglion of the facial nerve

Otalgia, unilateral facial paresis, vertigo, hearing loss, tinnitus

How well did you know this?

Not at all

Perfectly

EBV infects B lymphocytes by what receptor?

C3d aka CD21

How well did you know this?

Not at all

Perfectly

Adenovirus; name the types associated with

Respiratory infection

Hemorrhagic cystitis

childhood gastroenteritis

Respiratory infection: 1-14 &21

Hemorrhagic cystitis: 11 &21

childhood gastroenteritis: 40 &41

How well did you know this?

Not at all

Perfectly

Smudge cells

Decoy cells

Henderson Paterson

Smudge cells; Adenovirus

Decoy cells: BK virus

Henderson Paterson: Molluscum contagiosum

How well did you know this?

Not at all

Perfectly

Name the 6 category A agents of bioterrism

Variola (smallpox)

Hemorrhagic fever viruses

Bacillus antracis

Yersinia pestis

Clostridium botulinum

Fancisella tularensis

How well did you know this?

Not at all

Perfectly

Dane particle refers to?

Hep B, DNA virus; intact virion

How well did you know this?

Not at all

Perfectly

What type of HCV is mostly found in the US?

Type 1 (1a>1b)

1a more likely to develope resistence

How well did you know this?

Not at all

Perfectly

Antigenic drifts refer to?

Antigenic shifts?

Drifts: minor changes

Shifts: majore changes

How well did you know this?

Not at all

Perfectly

Hemadsorption positive viruses

Influenza A&B

Parainfluenza

Measles

How well did you know this?

Not at all

Perfectly

Name the associated virus

Croup

Subacute sclerosing paencephalitis

Tropical Spastic parapresis

ATLL - Adult T cell Leukemia/Lymphoma

Croup: Parainfluenza

Subacute sclerosing paencephalitis: Measles aka Morbillivirus

Tropical Spastic parapresis: HTLV1

ATLL - Adult T cell Leukemia/Lymphoma: HTLV1

How well did you know this?

Not at all

Perfectly

Walls

Gram + vs Gram - Vs Mycobacteria

Gram + : Thick peptidoglycan cell wall, no outer membrane

Gram - : Thin peptidoglycan cell wall, WITH outer membrane

Mycobacteria: Like Gram + but with mycolic acids (repels violet/iodine)

How well did you know this?

Not at all

Perfectly

Sheep agar

Most bacteria

Cholate agar

Fastidious baceria like Neisseria and Haemophilus

Buffered Charcoal Yeast Extract

Legionella Contains Cystine and Iron for growth

MacConkey and EMB Eosin Methylene Blue

Enteric Gram -

What antimicrobials (5) are in the Campy agar?

Drugs Campylobacter are resistent to: Cephalothin Amphotericin B Vancomycin Trimethoprim Polymyxin B

Hektoen Enteric agar is for?

Salmonella & Shigella

Thiosulfate-citrate-bile-salts-sucrose agar (TCBS) Whats special about it?

Vibrio Alkaline pH

Cefsulodin-Irgasan-Novobiocin again | (CIN)

Yersinia

Lim broth

Group B step Inhibits gram - with Colistin

Regan-Lowe medium

Bordetella Has cephalexin (bordetella is resistent)

Thayer-Martin media

Neisseria Has vancomycin, colistin, nystatin

Name the sugar in TCBS and CIN again

TCBS- Sucrose (fermenters turn yellow) CIN- Mannito (Fermenters have red center "bulls eye")

Name the organsim that grows at 25 -30 C 42C Grows at 37 but motile at 25 C, and multiple at 4C

25 -30 C : Yersinia and certain Pseudomonas (fluorescens, putida) 42C : Campylobacter jejuni/coli and Pseudomonas (except for the fluorescens, putida) Grows at 37 but motile at 25 C, and multiple at 4C: Listeria monocytoggens

Catalase + organsims

1. Staph 2. Pseudomonas 3. Aspergillus 4. Candida 5. Enterobacter 6. Bacillus 7. Campylobacter 8. Listeria 9. Staph 10. H. Pylori 11. Nocardia

S. Saprophyticus is resistent to?

Novobiocin

S. pneumonia is susceptible to?

Optochin P disk

PYR positive in?

Group A pyogenic strep Enterococcus Staph lugdunensis

Bile esculin test identifies?

Enteroccoci and Group D

Oxidase + organsims (7)

Pseudomonas Vibrio cholerae Neisseria Moraxella Campylobacter Helicobacter spp Legionella

Indole test for?

E. coli, Indole + Others: propionebacterium and pasturella

Urease positive oragnism

Brucella (Strong) Proteus (rapid) ureaplasma Nocardia Crytococcus Helicobacter pylori (Rapid)

Hippurate hydrolysis test (5)

+ (purple) in Campylobacter jejuni Listeria monocytogens Streptococcus agalactiae Gardenella Vaginalis Legionella pneumophila

Growth in lysozyme indicates?

Nocardia

What staph is resisent to vanc and shows beta hemolysis?

Staph Hemolyticus on a side note, Staph Aureus is also beta hemolytic

Name the organsim Gram + cocci in pairs Susceptible to bacitracin (A disk) PYR + Lancefield group A

Strep pyogenes

Name the organsim Lancet shaped, Gram + diplococci susecptible to optochin (p disk) bile susceptible lancet shaped, dipplococci

Strep pneumonia

Name the organsim gram + diplococci PYR + Bile + Grow in 6.5% NaCl Express Lancefield group D

Enteroccci

Enterococci is resistent to?

Cephalosporins Anti-staph penicillins (methicillin) Carboxypenicillin (ticarcillin) Penicillin (high MIC) Aminoglycosides

Name the organsim fastidious gram - cocci which requires incubation with CO2

Neisseria

Name the organsim Gram - cocci oxidase + Hockey puck sign resistent to?

Moraxella catarrhalis, resistent to penicillin (produce beta lactamase) freq cause of otitis media in children

Name the organsim Anaerobe spore forming gram + rods

Clostridium Bacillus is a aerobe, spore forming gram + rod

Name the organsim Anaerobe gram + rod, spore forming double zone of beta hemolysis on blood agar

clostridium perfringens

Name the organsim Anearobe gram + rod, spore forming cultured on Cycloserine Cefoxitin Egg Yolk Fructose Agar (CCFA) under strict anaerobic condidtions Colonies are yellow and fluoresce with UV light Smells like horse manure

C difficile

Severe C diff is associated with strain BI/NAP1/027 which has the mutated form of what gene?

tcdC gene

Name the organsim Anaerobe non spore forming, gram + rod molar tooth colonies aka cerebriform

actinomyces gram +, non spore forming anaerobe NOT acid fast (nocardia is acid fast)

Name the organsim Spore forming, Aerobic gram + cocci catalase +, non motile Medusa head colonies

Bacillus anthracis

Name the organsim Nonspore forming, aerobic gram + bacilli beta hemolytic with a narrow zone of hemolysis Catalase + Temperature dependent motility (25C) CAMP test -\> Rectangle Resistent to cephalosporins

Listeria monocytogens Use ampicillin

Name the organsim Nonspore forming Aerobic gram + rod non motile catalase - produce H2S intrinsically resistent to vancomycin

Erysipelothrix rhusiopathiae

Name the organsim Long thin filamentous beaded gram + rod Modified acid fast + Culture on BCYE, chalky white "musty basement" odor

Nocardia

Name the organsim gram +, coccobacili found in histiocytes Acid fast + Pink salmon colonies on blood

Rhodococcus equi

Name the organsim gram + rod Aerobic, non spore forming Tindsdale agar - Reduces potassium tellurite to metalic tellurite tonsils with pseudomembrane ELECK test for exotoxin

Corneybacterium Diptehriae

Name the organsim gram + rod aerobe, non spore forming foamy histiocytes in the duodenum PAS + AFB -

Tropheryma whipplei

Name the organsim Anaerobe gram - bacilli Grows in bile (black) Normal GI flora produce beta lactamase Use metronidazole Safety pin/bipolar look

Bacteroides fragilis Safety pin is a buzz word for Yersinia

Name the organsim anaerobe gram - rod, fusiform associated with Lemierre syndrome

Fusobacterium Lemierre syndrome : Tonsilitis complicated by septic thrombophlebitis of the internal jugular vein Vincent's angina

Of the enterobacteriacceae what reduce nitrate to nitrite?

All E coli, salmonella, citrobacter, shigella, klebsiella, proteus, yersenia, enterobacter

Of the enterobacteriacceae, what are strong lactose fermentors

E. Coli, Klebsiella, Enterobacter Citrobacter

Of the enterobacteriacceae, what produce Hydrogen sulfide

Salmonella Proteus Citrobacter Erysipelothric rhusiopathiae (gram +, non spore forming rod)

Name the Ecoli that does not ferment sorbitol Produce shiga like toxin

EHEC O157:H7

Name the Ecoli that is the cause of travelers diarrhea produce cholera like toxin

ETEC

Which shigella can also cause HUS? Causes postinfectious arthritis in HLA-B27 pts

HUS: S dysenteriae HLA-B27: S flexneri

Which klebsiella caues rhinoscleroma?

K rhinoscleromatis

Name the organism Gram - rod grows on MacConkey oxidase + ferments glucose Yellow on TCBS

Vibrio Cholerae Yellow on TCBS because it ferments sucrose

Name the organism Gram - rod grows on MacConkey oxidase + ferments glucose Green on TCBS Aquired through seawater

Vibrio parahemolyticus Green on TCBS because it does NOT ferment sucrose

Name the organism Gram - rod grows on MacConkey oxidase positive glucose non fermentor beta hemolytic can grow at 42C grape like odor Seen in Cystic fibrosis

Pseudomonas aeruginosa

Name the organism Gram - rod NO growth on MacConkey Requires cysteine Oxidase - Urease - beta lactamase + Transmitted by Amblyomma americanum (Lone star tick) Category A - agent of Bioterrorism

Francisela tularensis Use serology

Name the organism Gram - rod NO growth on MacConkey Intracellular pathogen grows on chocolate with CO2 Urease strongly positive Oxidase and Catalase Positive Category B- agent of bioterrorism Fever with lymphadenopathy, hepatosplenomegally, bad BO Spontaneous abortion Hepatitis with Granulomas endocarditis (principle source of mortality)

Brucella Abortus - cow sheep - melitensis swine- suis

Name the organism Gram - rod NO growth on MacConkey Requires nicotinic acid, cysteine, and methionine Small "mercury like" growth on Bordet Gengou or Regan Lowe media at 35C with CO2 supplement cause whooping cough

Bordetella pertussis

Name the organism Gram - rod NO growth on MacConkey Oxidase + Catalase + Stronly Indole + wound infection from animal bites

Pasteurella mutlocida

Name the organism Gram invisible NO growth on MacConkey White colonies on BCYE ID by latex agglutination or direct fluorescent ab or by urinary ag test

Legionella penumophila serogroup 1

Name the organism Tiny Gram - , seagull shaped NO growth on MacConkey Grows on CAMPY Oxidase + Catalase + Hippurate hydrolysis +

Campylobacter jejuni other campylobacter spp neg for hippurate

Name the organism spindle shaped, thin Gram - rod NO growth on MacConkey Chocolate with CO₂ Catalase - Oxidase - Indole - Dog/cat bite

Capnocytophaga canimorsus

Name the organsim Gram variable filamentous rod with moniliform swellling No growth on MacConkey Puffball on thioglycolate broth Causes rat bite fever

Streptobacillus moniliformis

Name the oragnism Gram - No growth on MacConkey Small colonies on chocolate Requires X and V

Haemophilus influenza

Name the oragnism Gram - No growth on MacConkey Small colonies on chocolate Requires V

H. parainfluenza

Name the oragnism Gram - No growth on MacConkey Small colonies on chocolate Requires X

H. ducreyi

Name the oragnism Gram - No growth on MacConkey require 48-72 hrs in CO₂ enriched enviorment on chocolate agar Causes endocarditis

HACEKs **_Oxidase neg_** Haemophilus Aggregatibacter **_Oxidase Pos_** Cardiobacterium hominis (indole +) Eikenella corrodens (Bleach smell, pits agar) Kingella (beta hemolytic)

Name the oragnism Gram - No growth on MacConkey Obligate intracellular pathogen causes Q fever Category B-bioterroism Granuloma found in liver and BM with classif fibrin ring appearance Diagnose by Serology or PCR

Coxiella burnetti

Weil disease

Triad of meningitis, hepatitis, and nephritis caused by Leptospira integrrogans Serology

associated with intestinal spirochetosis

brachyspira aalborgi

What causes syphillis? Whats the screening test?

Treponema pallidum Screen: treponemal specific test (FTA-ABS, TP-PA), confirm with non treponemal test (RPR and VDRL)

What caues lyme disease? Coinfection with? Vector?

Borrelia burgdorferi Babesia and Anaplasma Ixodes tick

Whats the gold standard for Chlamydiae diagnosis?

growth on McCoy cells NAAT available

Name oragnism which causes Rocky Mounted spotted fever Vector?

Rickettsiae rickettsii Dermacenter variabilis aka American Dog tick

Ehrlichia chaffeensis infects? Anaplasma phagocytophilum infects?

Ehrlichia chaffeensis infects; Monocytes - Amyblomma americanum Anaplasma phagocytophilum infects: granulocytes - Ixodes

Name organism and vector Trench fever

Bartonella quintana - Pediculus humanus

Name organism and vector Oroya fever and verruga peruana

Bartonella bacilliformis - sand fly Lutzomyia phlebotomine

Name organism and vector cat scratch disease bacillary angiomatosis

bartonella henselae

Micro Flashcards

(101 cards)