MICRO BIO LAB QUIZ 4 Flashcards
(23 cards)
These are transferred from one culture medium to another by subculturing
Microorganisms
Using a specific procedure and aseptic technique
Subculturing
it is inoculated or introduced into various forms of culture media in order to keep them alive and to study growth
Bacteria
it must be done without introducing unwanted microbes or contaminants into the media
inoculation
it is used in microbiology to exclude contaminants
Aseptic techniques
media are sterilized or rendered free from all forms of life prior to use
all cultures
it is usually accomplished using autoclave during the media preparation?
Sterilization
what are the containers used in culture media?
test tubes and petri plates
it should not be opened or left opened until the culture media is ready for inoculation test
test tubes and petri plates
Transfer and inoculation are usually performed with?
sterile, heat resistant, non-corroding nichrome wire
what is attached to an insulated handle?
non-corroding nichrome wire
when the end of the wire is bent into a loop it is called?
inoculating loop
when the wire is straight it is called?
inoculating needle
what are the special purposes for transferring culture? depending on the form of the medium and technique
sterile cotton swab
pipette
glass rod
syringe
what ate the materials
inoculating loops
inoculating needles
inoculum
bacterial culture
alcohol lamp
incubator
nutrient agar plates
agar slants
agar deep
nutrient broths
what are the samples of inoculum?
sample from soil, water, feces, urine, sputum
What are the kinds of bacterial culture used in this experiement?
Staphylococcus aureus and Escherichia coli
how many degrees Celsius must the alcohol lamp be set
37 degrees Celsius
In simple streak how many hours must the petri dish be incubated?
24-48 hours at 37 degrees Celsius
in Incubation of inoculated culture media, how many hours must the agar plate and agar slant and deep be inoculated?
18-24 hours
when we say agar plate media what must be the position?
upside down?
when we say agar slant and deep what must be the position?
upright position on the test tube rack
What is the title of the experiment?
Inoculation of culture media: Aseptic Transfer Technique
