Micro Lab Quiz 1 Flashcards Preview

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Flashcards in Micro Lab Quiz 1 Deck (20)
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1
Q

Baccilus

A

Long rod like image under scope

2
Q

Spirilli

A

spiral looking circles under scope

3
Q

Cocci

A

circular blobs under scope

4
Q

What type of microscope are we using in class?

A

Light Microscope

5
Q

How much does your ocular lens magnify the object

A

10x

6
Q

Total Magnification

A

= 4x * 10x = 40X

7
Q

RP Equation

A

550 nm/ 2 * numerical aperture

8
Q

What is the purpose of oil used in 100x

A

light bends as it goes from sample to air, oil prevents light scatter

9
Q

What is the smallest size object that can be clearly seen

A

220 nm, maximum resolution

10
Q

Microscope part- Condenser

A

moves light up and down

11
Q

Why do we stain bacteria prior to microscopic examination?

A

increase contrast to be more easily seen

12
Q

What is the purpose of heat fixing?

A

to kill the organism, make it adhere to the slide, and alter the organism so that they accept stains

13
Q

Gram Stain

A

differentiating bacterial species between gram-positive and gram-negative. Based on Cell wall composition.

14
Q

Peptidoglycan

A

is a polymer consisting of sugars and amino acids that forms a mesh-like layer outside the plasma membrane of bacteria (but not Archaea), forming the cell wall.

15
Q

Gram positive color

A

purple

16
Q

Gram negative color

A

colorless, thinner Peptidoglycan.

17
Q

Acid Fast Stain (differential)

A

Mycobacteria from other bacterial genera

18
Q

Capsule Stain (structural)

A

capsule or slime layer

19
Q

Endospore stain (Structural)

A

whether bacteria produces spore

20
Q

Flagella stain (structural)

A

have flagella and are motile