Microbial Growth & Nutrition Flashcards Preview

Microbio & Imm. > Microbial Growth & Nutrition > Flashcards

Flashcards in Microbial Growth & Nutrition Deck (17):
1

On an agar plate, a discrete colony arising from a single parent cell is known as:

Clones

2

What are the 6 major elements in an organism?

-Carbon
-Hydrogen
-Nitrogen
-Oxygen
-Sulfur
-Phosphorus

3

Obligate aerobes grow ___ oxygen, while obligate anaerobes grow ____ oxygen (with or without)

With, without

4

Give an example of a microaerophile (grow best in low oxygen concentrations)

Streptococcus

5

Anabolism/growth often ceases because of insufficient ____ (element)

Nitrogen

6

Name the 3 physical requirements for growth of microorganisms

1. Temperature
2. pH
3. Osmolarity

7

___ can multiply in the refrigerator

Psychrophiles

8

____ grow best inside the human body (37 degrees Celsius. These are pathogens)

Mesophiles

9

With respect to pH, what group do most human pathogens belong to? (They grow best around neutral pH)

Neutrophiles

10

Helicobacter pylori is found in the stomach, what group does it fit in in regards to pH?

Neutrophile (It was once thought to be an acidophile, but thats not the case)

11

A common isolation technique for obtaining pure cultures:

Streaking (streak plate)

12

A polysaccharide from marine algae

Agar

13

Best method for long-term preservation of bacterial cultures?

Lyophilization (freeze-drying)

14

Serial dilution and viable plate counts, membrane filtration, and microscopic counts are all ___ methods for measuring microbial reproduction

Direct

15

Dry weight and turbidity (A600) are ____ methods for measuring microbial growth

Indirect

16

Quick and dirty way to measure microbial growth?

Spectrophotometer

17

List the 4 phases of microbial growth from start to finish when a medium is inoculated:

1. Lag phase
2. Log phase (exponential growth)
3. Stationary phase
4. Death phase