Microbial Lifestyles and Metabolism (Dr. Kreft): Exponential Growth Flashcards Preview

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Flashcards in Microbial Lifestyles and Metabolism (Dr. Kreft): Exponential Growth Deck (22):
1

What is the definition of growth?

- forming two cells from 1
- increase in both mass and number
- absolutely necessary for survival
- application of growth = stop growth of microbes or exploit it

2

Describe some E. coli growth facts!

- 20 min generation time of cell under optimal conditions
- 40 min DNA replication
- 20 min from end of replication to cell division
- They have to start replication 60 min before division, every 20 min, so that several rounds of replication are running simultaneously. Bacteria usually have one chromosome with one origin of replication
- generation time in colon = 12 hours or more

3

In terms of number of cells and generation describe what the following numbers mean:
2^0 -> 2^1 -> 2^2 -> 2^3 --> 2^4

- 1 cell, generation 0
- 2 cells, generation 1
- 4 cells, generation 2
-8 cells, generation 3
- 16 cells, generation 4

4

Describe N= N0 * 2^n

- Number of cells after n generations (general)
-N= number of cells
- N0= number of cells at the beginning (time 0)
- n= number of generations
- generation time = time/number of generations (g=t/n)
- t= time (since t0)

5

How many offspring from a single E. coli cell in 2 days?
- generation time = 20 min

- 3 generations per hour
- 2 days = 48 hours
- 3x48= 144 generations
- N= 2^144= 2.2 x10^43 cells in 2 days
**estimated 10^30 prokaryote cells on earth....so this is unlikely
Mass: one cell 10^-12 g... 2.2x10^43 cells * 10^-15kg/cell = 2.2 10^28kg = ~ 4,000 times the mass of the earth (6x10^24kg)

6

Explain why exponential growth does not go on forever!

- run out of nutrients (just a single nutrient lacking will stop growth)
- waste products can be toxic( acetic acid, ethanol..)
- predation/phages( in nature, not in lab batch culture)
- space ( usually not the first resource to run out)

7

Why does exponential growth occur?

- because growth is proportional to the current cell number (low numbers = continuous growth is possible)

8

Examples of exponential growth?

- microbes (not with mycelium)
-animal/plant cells in tissue culture --> cancer

9

What is specific growth rate?

- growth rate per biomass
- growth rate/ biomass

10

Growth of ___, not ___

biomass
number
**growth rate (slope) increases exponentially and so does biomass on a graph with x= time and y= biomass
**growth rate is proportional to the current biomass
** twice as much biomass grows twice as fast. In other words, growth rate is proportional to biomass . Specific growth rate is growth rate per biomass

11

For animal/human populations, the specific growth rate is called?

per capita growth rate

12

Explain the change of biomass per time
dX/dt= u*X

- x is biomass, t is time, dX, dt are change of X, t, u is specific growth rate
- the specific growth rate is the proportionality constant
- that is a differential equation expressing rate of change
- growth rate= specific growth rate *biomass

13

What is the solution for the change of biomass per time differential equation?
dX/dt= u*X

- X= X0 *exp(u*t) or X= X0 e^ut
-x = biomass
- X0= biomass at t0
- u = specific growth rate (dimension per time)
t= time
- e = euler's number = 2.71828
**inverse of the natural logarithm ln(x)= 1, ln(e^x)= x

14

Explain balanced growth!

- all components of the cell increase in proportion
- biomass, protein, number of cells, all increase at the same rate
- the composition of the cell does not change
- this is characteristic for exponential growth phases

15

Cell number based measures/concepts:
- generation time?
- division rate?

- g
- v= 1/g

16

Biomass based measures/concepts:
- doubling time?
- growth rate?

- td
- u= ln(2)/td

17

What is the simple relationship between growth of number and biomass in balanced growth?

- generation time g= doubling time td

18

Exponential growth will only occur if?

- the specific growth rate is constant
- sufficient and necessary conditions
L> two ways this can be achieved : enviornment is constant (ex chemostat)
or the enviornment changes but the bacteria does not notice it (excess substrate in batch culture)

19

How is it possible for exponential growth to occur via the bacteria not noticing changes in the environment?

- a decrease of substrate concentration does not lead to a decrease in specific growth rate as long as substrate concentration is saturating (in excess)
- the saturating curve is known as Monod kinetics

20

Do cultures grow exponentially if conditions are constant, whether optimal or not?

YES

21

Is exponential growth, growth at any specific growth rate?

YES

22

Can exponential growth be fast or slow?

YES - both