Flashcards in Microbiology Deck (48):
What shape are cocci organisms?
What shape are bacillus organisms?
Long and thin
What colour are gram positive organisms when stained?
What colour are gram negative organisms when stained
Which group produces exotoxins?
Which group produces endotoxins?
Describe a gram positive cell wall
Small phospholipid layer and large peptidoglycan layer
Describe a gram negative cell wall
Peptidoglycan layer between two phospholipid layers
How long does microscopy take?
How long does PCR take?
How long does culture on agar take?
How long does serology take?
How long does TB culture take?
Which organisms are gram positive cocci?
Which organisms are Gram positive bacilli?
Which organisms are gram negative cocci?
which organisms are gram negative bacilli?
Give examples of gram negative anaerobic bacilli
Give examples of coliforms
Gut commensals - E.coli, Klebsiella, Proteous
Gut pathogens - salmonella, shigella, E. coli 0157
Give examples of strict aerobes
Give examples of curved gram negative bacilli
Which organism is differentiated by type using haemolysis?
Partial = alpha
Complete = beta
Non-haemolytic = gamma
gram positive aerobic clustered cocci, use coagulase test to distinguish
Positive = golden
What is MSSA sensitive to?
What is the first line antibiotic for coliform infections?
What is transformation?
When DNA from dead bacteria can be taken up by living bacteria and be incorporated
What is conjugation?
bacterial sex where plasmid DNA can be transfered
What is transduction?
Viruses that infect bacteria and can transfer DNA from one to another
Which antibiotics act on the bacterial cell wall?
Which antibiotics inhibit protein synthesis?
Clindamycin and chloramphenicol
Which antibiotics act on bacterial DNA?
How do certain antibiotics inhibit protein synthesis?
attaching to bacterial ribosomes which are different to mamallian ribosomes
How do certain antibiotics act on the cell wall?
Inhibit cell wall synthesis by preventing cross linkage of peptidoglycan
Why is amoxicillin often less effective?
Many organisms produce beta-lactamaze which is an enzyme that destroys amoxicillin
Which antibiotics are used for coliforms?
Which antibiotics are used for anaerobes?
Which antibiotics are used for Enterococcus?
Gentamicin and metronidazole
Where are exotoxins produced?
Produced inside the cell
Where are endotoxins produced?
Part of the bacterial cell wall
Name the 4 C's
How is streptococcus identified?
Strep. Pneumoniae and viridian's
Group A and B
Gamma haemolysis/no haemolysis
If staphylococcus is coagulase positive what bacteria is present?
If staphylococcus is coagulase negative what bacteria is likely to be present?