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Flashcards in Microbiology--> *stains and general characteristics Deck (16):
1

what do gram + have in their outer cell membrane that is a virulence factor

Lipoteichoic acid --> can stimulate cytokine release (IL-1, IL-6, TNF)

2

what do gram -- have in membrane that is a virulence factor? which part is it found in

LPS (an endotoxin)--> found in outer cell membrane

3

what is found in the periplasmic space of gram -- organisms? what two structures is this space between

1. β-lactamases

*periplasmic space is b/n the cytoplasmic membrane and the outer membrne

4

mediates adherence to surfaces, especially foreign surfaces like indwelling catheters or teeth

glycocalyx

5

adherence of bacteria to cell surfaces

fimbriae

6

gives rigid support protects form osmotic pressure

peptidoglycan

7

6 organisms that dont gram stain well

("These Rascals May Micrscopically Lack Color")

1. Treponema
2. Rickettsia
3. Mycobacteria
4. Mycoplasma
5. Legionella pnemophila
6. Chlamydia

*legionella, mycoplasma, and rickettsia are intracellular primarily

8

8 bacteria with capsules

("Even Some Pretty Nasty Killers Have Shiny Bodies")

1. E coli (some)
2. Salmonella typhi
3. Pseudomonas aeriginosa
4. Neisseria meningitidis
5. Klebsiella pnem.
6. H. flu B
7. Strep pneum.
8. group B strep

9

what 3 organisms cause high mortality in apslenic patients

all encapsulated, but especially:
1. Strep pneum
2. H. flu B
3. Neisseria menigitidis

10

5 organisms that can be visualized with Giemsa stain

("Certain Bugs Really Try My Patience")

1. Chlamydia
2. Borrelia b.
3. Rickettsiae
4. Trypansosomes
5. Plasmodium

11

PAS can be used for what organism

T. whipplei

12

Ziel-Neelson stain stain what kind of organisms, two examples

acid fast:
1. Mycobacteria
2. Nocardia

13

India ink stains what organism

Cryptococcus neoformans (encapsulated yeast)

14

Silver stain can stain what three organisms

1. Pneumocystis jirovecii
2. Legionella
3. H. pylori

15

no cell wall, has sterols

Mycoplasma

16

what are Mycobacterial cell walls made of

mycolic acids and lipids

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