Microbiology--Virology Flashcards Preview

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Flashcards in Microbiology--Virology Deck (56):
1

Live attenuated vaccines:

induce humoral and cell-mediated immunity: smallpox, yellow fever, chickenpox, sabin polio virus, MMR, intranasal influenza

2

Killed vaccines

induce only humoral immunity: rabies, influenza, salk polio, HAV

3

Recombinant vaccines:

HBV (HBsAg), HPV (6/11/16/18)

4

which DNA viruses are dsDNA

all except parvovirus (ssDNA)

5

which DNA viruses are linear?

all except papilloma, polyoma and hepadenaviruses (circular)

6

which RNA viruses are ssRNA?

all except reoviridae (dsRNA)

7

which RNA viruses are positive stranded?

retrovirus, togavirus, flavivirus, coronavirus, hepevirus, calicivirus, picornavirus: I went to a retro toga party where I drank flavored Corona and ate hippy california pickles.

8

which purified nucleic acids of viruses are infection? general rules?

most dsDNA (except poxvirus/HBV) and +strand ssRNA viruses are infection. -strand ssRNA and dsRNA are not infectious and require polymerases contained in complete virion.

9

DNA viruses replicate where?

nucleus (except poxvirus--carries its own DNA dependent RNA polymerase)

10

RNA viruses replicated where?

cytoplasm (except influenza and retroviruses)

11

Naked viruses are which:

Papillomavirus, adenovirus, parvovirus, polyomavirus, calicivirus, picornavirus, reovirus, hepevirus: give PAPP smears and CPR to a naked Heppy (DNA = PAPP, RNA = CPR)

12

what are the DNA viruses:

HHAPPPPy: hepadna, herpes, adeno, pox, parvo, papilloma, polyoma

13

what are the two polyoma viruses:

JC virus: PML in HIV; BK virus: transplant patients targeting kidney

14

flesh colored dome lesions with central umbilicated dimple

molluscum contagiosum--poxvirus

15

latent in trigeminal ganglia

HSV-1

16

latent in sacral ganglia

HSV-2

17

latent is dorsal root or trigeminal ganglia

VZV (HHV-3)

18

infects B cells, reactive cytotoxic T cells seen on smear

EBV (HHV 4)

19

Which diseases is EBV ass. with?

hodgkin lymphoma, endemic burkitt lymphoma, nasopharyngeal Ca

20

latent in mononuclear cells, owl eye inclusions

CMV (HHV 5)

21

roseola--exanthem subitum; high fevers that can cause seizures followed by diffuse macular rash

HHV6

22

dark/violaceous flat and nodular skin lesions

Kaposi sarcoma--HHV8, endothelial growths

23

Tzank test

smear of opened skin vesicle to detect multinucleated giant cells

24

intranuclear Cowdry A inclusions

a cell infected with HSV

25

What are the negative stranded RNA viruses?

Arenaviruses, Bunyavirus, Paramyxoviruses, Orthomyxovirus, Filovirus, Rhabdovirus; Always Bring Polymerase Or Fail Replication; All have RNA-dependent RNA polymerase

26

Which are the segmented viruses?

All RNA viruses. BOAR: bunyavirus, orthomyxoviruses (influenza), arenaviruses, reoviruses

27

What are some picornaviruses?

Poliovirus, Echovirus, Rhinovirus, Coxsackievirus, HAV (PERCH)--RNA is translated into 1 large polypeptide that is cleaved by proteases into functional viral proteins

28

high fever, black vomitus, jaundice

flavivirus--yellow fever virus--"flavi" = yellow; abovirus via Aedes mosquito

29

most important global cause of infantile gastroenteritis, acute diarrhea esp during winter in daycares

rotavirus; segmented dsRNA virus (reovirus); villous destruction with atrophy leads to hyponatremia and hypokalemia

30

what proteins does influenza virus have to help in virulence?

hemagglutinin to promote viral entry, neuraminidase to promote progeny release

31

fever, postauricular and other lymphadenopathy, arthralgias, fine rash

rubella vrus, togavirus

32

"blueberry muffin" appearance in newborn

congenital rubella infection indicative of extramedullary hematopoesis

33

what diseases to paramyxoviruses cause?

Parainfluenza (croup); RSV (bronchiolitis in babies; ribavirin); Measles, Mumps

34

what is common to all paramyxoviruses that aids in their virulence?

all make F (fusion) protein that causes respiratory epithelial cells to fuse and form multinucleated cells--Pavalizumab is monoclonal ab against F protein (used to prevent RSV infection in premature infants)

35

koplik spots and descending maculopapular rash

measles virus--paramyxovirus, subacute sclerosing panencephalitis, cough/coryza/conjunctivitis; use vit Ato prevent exfoliative dermatitis in malnourished children

36

paratotitis, orchitis, aseptic meningitis

Mumps paramyxovirus

37

bullet shaped virus

Rabies--negi bodies found in purkinje cells of cerebellum and in hippocampal neurons--retrograde movement up nerve axons

38

HIV env protein

gp120 (docking glycoprotein, attachement to host CD4+ T cells) gp41 (transmembrane glycoprotein, fusion and entry)

39

gag HIV

p24--capsid protein

40

HIV pol

reverse transcriptase, aspartate protease, integrase

41

which receptors on which immune cells does HIV bind to

early: HIV binds to CCR5 on CD4+ T cells; late: HIV binds CXCR4; binds CCR5 and CD4 on macrophages; homozygous CCR5 mutation gives immunity to HIV

42

HIV positive adult: low grade fevers, cough, HSM, tongue ulcer, CD4 < 100

histoplasma capsulatum (only causes pulmonary symptoms in immunocompetent hosts)

43

HIV positive adult: fluffy white cottage cheese lesions

C albicans: mouth if CD4 < 400; esophageal CD4 < 100

44

HIV positive adult: hairy leukoplakia

EBV

45

HIV positive adult: superficial vascular proliferation with neutrophilic inflammation

bartonella henselae

46

HIV positive adult: chronic water diarrhea with acid fast cysts

cryptosporidium CD4 < 200

47

HIV positive adult: encephalopathy

JC virus, CD4 < 200

48

HIV positive adult: meningitis

cryptococcus, CD < 50

49

HIV positive adult: retinitis and esophagitis

CMV, CD4 < 50

50

EBV + HIV associations:

non hodgkin large cell lymphoma (often on oropharynx) and primary CNS lymphoma

51

HIV positive adult: cancer on anus

SCC, HPV, men who have sex with men

52

HIV positive adult: superficial neoplastic proliferation of vasculature with lymphocytic inflammation

Kaposi HHV8

53

HIV positive adult: pleuritic pain, hemoptysis, infiltrate

aspergillus fumigatus

54

HIV positive adult: CD4 < 200, ground glass on imagine

PCP

55

HIV positive adult: pneumonia, CD4 > 200

S pneumoniae

56

HIV positive adult: TB like disease; CD4 < 50

MAC