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Flashcards in Microscopy Deck (19)
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0

State the hierarchy of measurement units

M,mm,um,no

1

Define tissue

A collection of cells specialised to perform a particular function e.g. Epithelia

2

Explain the value of histology in diagnosis

Can give certainty, biopsy is proof

3

Define biopsy

removal of small piece of tissue

4

What is curettage used for?

Endometrial lining of uterus

5

What is smear used for?

Cervix,Buccal

6

What is needle used to sample?

Brain, breast, liver

7

What is direct incision used to sample?

Skin, mouth, larynx

8

What is endoscopic used for?

Lung, intestine, bladder

9

What is transvascular used to sample?

Heart and liver

10

Why is tissue fixed?

Macromolecules cross linked
Preserve cellular structure
No auto lysis or putrefaction

11

List common fixatives

Gluteraldehyde
Formaldehyde
Dehydrated with ethanol, cleared with toluene, wax

12

How do shrinkage artefacts occur?

During slide preparation dehydration and rehydration leads to abnormalities

13

How does h and e stain work?

Haemotoxin stains acidic components purple e.g. DNA RNA
Eosin stains basic components pink e.g. Proteins, fibres

14

How does periodic acid shift stain?

Stains glycoproteins magenta

15

What is phase contrast and its advantages?

Used inteferance effects from two combining light waves
Can enhance image of Unstained cells

16

What is dark field and its advantages?

Excludes The U scattered beam from the image
Can use on Unstained, detects syphyllis and malaria

17

What is flourescent and its advantages?

Targets molecules of interest with flourescent ab
Can use multiple stains on one sample

18

What is confocal and its advantages?

Tissue labelled with one or more fluorescent particles
Eliminates flare, 3d from 2d, living specimens