Microscopy + Visualisation Flashcards
Light bends when it passes through a lens. This is called…
Refraction
Using the light microscope you can see…
Cells!
The light microscope gives a …
Enlarged inverted image
The lens in the eyepiece of the light microscope is called the…
Ocular lens!
What controls how much light passes through the stage?
Diaphragm
Which objective lens should you use when you FIRST start focusing your specimen?
4x
Resolution is determined by…
Wavelength of radiation and NA of objective
Limit of resolution of the light microscope is…
200nm
The NA or Numerical Aperture describes the… of an objective.
Ability to collect light
Which objective must NEVER be used with the coarse adjustment knob?
100x
What is the function of the condenser lens?
Focuses light through the specimen!
A microscope has 10x ocular lens and 40x objective, what is the total magnification?
400x
10 x 40 = 400!
Which type of microscope would allow you to see the 3D SURFACE of bacteria and viruses?
Scanning electron microscope
Which type of microscope gives the greatest magnification?
Electron microscope
The MAIN reason that the electron microscope gives a BETTER resolution than the light is…
The shorter wavelength of an electron beam
… waves have the highest / longest wavelength
Micro
Which type of microscope uses UV light?
Fluorescence microscope
On the objective lens what is number written next to the word oil?
Numerical Aperture
What is the 2nd number written on the objective?
Coverslip thickness
What is the FIRST step of tissue preparation?
Fixation!
What is the most common fixative used?
How does it preserve a specimen?
Formalin
By cross linking proteins!
After embedding the specimen, what is the first thing you need to do before you stain it?
Cut it into thin sections
The organic dye Eosin is used to stain…
The cytoplasm
In Immunohistochemistry, the DIRECT method used a primary and secondary antibody.
True or false?
FALSE
