MIDTERM 2 Cytochrome C Flashcards Preview

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Flashcards in MIDTERM 2 Cytochrome C Deck (7)
1

What does respiratory catabolism involve

They involve enzymes that rapidly break down (oxidize) compounds such as sugars via the EMP pathway and teh TCA cycle.

2

What is oxidative phosphorlyation?

Electrons are passed from one cytochrome to another and with each step they transfer some of their energy to the phosphorylation of ADP to ATP

3

What is the purpose of the terminal electron acceptor?

it removes the energy spent electrons, which are no longer of any use to the cell

4

What is Needed for both catalase and cytochrome c test?

A fresh culture

5

What will the presence of cytochrome C prove?

a microbe has the capacity for respiratory catbolism

6

How can we identify for Cytochrome C oxidase test work?

1. add Tetramethyl-p-phenylenediamine ( colorless reagent in reduced state_
2. the reagent will be oxidize when the electrons are transferred to cytochrome C Changing its color to a dark purple compound known as wuster blue.

7

Positive result for cytochrome C test?

Deep violet/purple color within 10 seconds