Module 14

Question 1

Denaturation in a PCR reaction is caused by _____ and the _____ bonds are broken.

Answer 1

temperature changes; hydrogen

Question 2

In England, the Home Office estimated that approximately ___% of the samples at a crime scene that are analyzed help secure a criminal conviction

Answer 2

50%

Question 3

What is the name of the PCR phase that uses Taq DNA polymerase? One word, spell it correctly, and don’t include any other words or punctuation.

Answer 3

extention

Question 4

_______ are translated to produce _______.

Answer 4

exons; proteins

Question 5

If the odds of a STR match at locus 1 is 1 in 10 and at locus 2 is 1 in 20, what is the probability of another person having the same STR pattern?

Answer 5

1 in 200

Question 6

During electrophoresis, the DNA moves towards the ____ pole because the DNA has an overall ___ charge.

Answer 6

+;-

Question 7

What cell type in a human blood sample contains DNA?

Answer 7

white blood cells

Question 8

_______ light is used to visualize DNA electrophoresis gels that have been stained with ethidium bromide?

Answer 8

UV

Question 9

How much of the human genome actually codes for physical traits?

Answer 9

less than 2%

Question 10

What are STR’s?

Answer 10

Short tandem repeats. Sections of DNA arranged back to back repition

Question 11

What is polymorphism

Answer 11

the variation in STR alleles

Question 12

What are the STR’s used in forensics called?

Answer 12

loci or markers

Question 13

how is DNA processed in a lab?

Answer 13

Polymerase chain reaction

Question 14

What are the steps of PCR?

Answer 14

Denaturation, annealing (primers match up with bases), extension (uses taq polymerase to put together DNA molecule)