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Microbiology:Bacteriology > Mycobacteria > Flashcards

Flashcards in Mycobacteria Deck (66):
1

cell wall content of Mycobacteria

N-glycol muramic acid

2

appearance of Mycobacteria when incomplete staining is done

Beaded appearance

3

pH of Mycobacteria

6.5-6.8

4

Mycobacterium tuberculosis complex

Mycobacterium tuberculosis
Mycobacterium bovis
Mycobacterium africanum
Mycobacterium canetti
Mycobacterium microti

5

colonial morphology of M. tuberculosis

cauliflower colonies

6

used for BCG vaccine

M. bovis

7

smooth strain of M. tuberculosis

M. carnetti

8

chronic granulomatous infection

tuberculosis

9

airborne nuclei in tuberculosis

1-5 um

10

characteristic of tubercle

cheesy like consistency

11

calcified tubercle that can be identified in chest x-rays

Ghon complexes

12

tuberculosis spondylitis

Pott's disease

13

extrapulmonary tuberculosis

miliary tuberculosis

14

classification of Mycobacteria

Runyon's classification

15

photochromagens

"simi is a marine that lived in asia then kansas"
M. kansasii
M. asiaticum
M. marinum
M. simiae

16

scotochromagens

"gordon tasted a flavored scroful by zul in a xenopi"
M. szulgai
M. gordonae
M. scrofulaceum
M. flavescens
M. xenopi

17

non-photochromagens

"bloody birds in my terrace cause ulcers in my GIT"
M. avium complex
M. ulcerans
M. terrae complex
M. gastri
M. haemophilum

18

rapid growers

M. fortuitum
M. chelonae
M. smegmatis
M. phlei
M. abcessus
M. mucogenicum

19

characteristics of photochromagens

cream/buff yellow orange under the light

20

characteristics of scotochromagens

yellow orange under the light and dark

21

most common non-M. tuberculosis in AIDS patients

M. avium complex

22

causative agent of Johne's/Crohn's disease

M. avium subspp. paratuberculosis

23

colonial morphology of M. kansasii

rough colonies with dark centers and waxy edges

24

causative agent of swimming pool granuloma with red and blue nodule

M. marinum

25

causative agent of Buruli ulcer

M. ulcerans

26

colonial morphology of M. xenopi

bird's nest appearance

27

Mycobacterium terrae complex

M. terrae
M. triviale
M. nonchromogenicum

28

most common rapidly growing mycobacteria

M. fortuitum

29

associated with outbreaks from DPT polio vaccines, histamine and lidocaine injection

M. chelonae

30

M. smegmatis group

M. smegmatis sensu strict
M. goodie
M. wolinskyi

31

best specimen for Mycobacterium tuberculosis

sputum

32

criteria for sputum

<10 epithelial cells
>25 pus cells

33

best specimen for non-Mycobacterium tuberculosis

bronchoscopy

34

specimen for M. avium in patient with AIDS

feces

35

number of organisms to obtain a positive result

5,000 to 10,000 organisms/ml

36

sensitive, reliable and specific stain for M. tuberculosis

Auramine-rhodamine

37

positive stain for auramine-rhodamine

bright yellow, orange against a dark background

38

number of field counted before reporting as negative

300 OIO fields

39

egg-based medium

Lowenstein-Jensen
American Thoracic Society
Petragnani
Wallenstein

40

serum-based TRANSPARENT media

Middlebrook 7H10
Middlebrook 7H11
Mitchison's medium

41

Liquid broth

Middlebrook 7H12
Middlebrook 7H13

42

most common medium for M. tuberculosis

LJ medium

43

medium for CSF and bone marrow samples

American Thoracic Society

44

medium for heavy contamination

Petragnani

45

medium for the growth of M. avium

Wallenstein

46

most common decontaminant and digestion agent

2-4% sodium hydroxide

47

decontamination agent with 5% concentration mostly used for sputum

Oxalic acid

48

decontaminant and digestive agent for sputum with large numbers of bacteria

Zephiran trisodium phosphate

49

decontaminating agent in zephiran trisodium phosphate

zephiran

50

digestive agent in zephiran trisodium phosphate

trisodum

51

buffer agent in zephiran trisodium phosphate

phosphate

52

decontaminant and digestive agent for sputum for prolonged shelf life (8 days)

1% pyridium chloride

53

most common biochemical test for Mycobacterium tuberculosis (positive result)

Niacin (nicotinic acid) test

54

indicator system in nitrate reduction test for Mycobacterium tuberculosis

zinc

55

test to differentiate M. tuberculosis and M. bovis

thiophene-2-carboxylic acid hydrazide (T2H)

56

principle of thiophene-2-carboxylic acid hydrazide (T2H)

inhibition

57

positive microorganism in thiophene-2-carboxylic acid hydrazide test

M. bovis

58

Mycobacteria that invades the peripheral nerve

Mycobacterium leprae

59

morphology of M. leprae

cigar pocket/pocket fence appearance

60

culture media for M. leprae

Armadillo

61

definitive test for M. leprae

foot pads of mice

62

disease caused by M. leprae

Hansen's disease/ leprosy

63

characteristics of tuberculoid leprosy

localized
benign
non-progressive
loss of sensation
neural

64

characteristics of lepromatous leprosy

malignant
progressive
loss of facial features

65

test for to differentiate the two types of leprosy

Lepromin

66

positive for lepromin

tuberculoid/neural leprosy