Mycology

Question 1

Heterotrophs

Answer 1

Digest complex organic material (plant/animal) food internally

Question 2

Heterotrophs may be what?

Answer 2

Parasitic or Saprophytic

Question 3

Parasitic

Answer 3

Cause harm to the plant/animal host it is using for food

Question 4

Saprophytic

Answer 4

Feed on dead/dying material

Question 5

Where may you see saprophytic fungi?

Answer 5

Fallen trees

Question 6

Yeast

Answer 6

Single-celled and reproduces by budding

Question 7

Hyphae

Answer 7

Multicellular fungal organisms

Microscopic

Question 8

Mycelium

Answer 8

Large branched webbings

Composed of hyphae

Question 9

What are the supplies needed for collection & examination of fungal samples

Answer 9

1. Forceps
2. Swabs
3. Lactophenol cotton blue
4. Potassium hydroxide solution
5. Culture media (DTM and/or Sabouraud agar)

Question 10

Sabouraud Dextrose

Answer 10

Commonly found in most clinics
Does not contain an indicator

Question 11

What stain confirms identity of fungal organisms?

Answer 11

Lactophenol cotton blue

Question 12

What are the common fungal species?

Answer 12

Microsporum canis
Microsporum gypseum
Trichophyton mentagrophytes

Question 13

Microsporum canis

Answer 13

Spores look like canoes and are pointed at the end

Question 14

Microsporum gypsum

Answer 14

Similar to Microsporum canis but is more rounded does nit have the point at the end

Question 15

Trichophyton mentagrophytes

Answer 15

Large and small spores

Question 16

What percentage of cases with Microsporum canis show evidence of fluorescence?

Answer 16

50%

Question 17

How long should a Woods lamp be warmed up for?

Answer 17

5 mins

Question 18

What are the 3 ways to test for Dermatophyte testing?

Answer 18

Wood’s lamp
Dermatophyte test Medium
Rapid sporulation medium

Question 19

Wood’s lamp

Answer 19

Infected hairs will glow in a dark room using fluorescence light

Question 20

Dermatophyte Test Medium (DTM)

Answer 20

Culture media will turn in red in the presence of most dermatophytes

Question 21

Rapid Sporulation medium (RSM)/Enhanced sporulation medium (ESM)

Answer 21

Culture media will turn teal in the presence of most dermatophytes

Question 22

What color does a positive result for DTM yield?

Answer 22

Red

Question 23

If the color change occurs is that 100% positive?

Answer 23

No. Confirm with microscopic examination

Question 24

What agar types are used for non-dermatophytes typically?

Answer 24

Bismuth-Glucose-Glycine Yeast (Biggy)
Blood agar
Sabouraud dextrose agar

Question 25

How do you do prepare a slide for microscopic evaluation of fungi?

Answer 25

Collect mycelium from the media with sticky side of clear cellophane tape

Question 26

What stain do you place on the slide for microscopic evaluation?

Answer 26

Lactophenol cotton blue

Question 27

How do you do evaluate fungus microscopically?

Answer 27

After placing the lactophenol cotton blue onto the slide, place the tape onto the stain and examine on 10x and 40x