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Flashcards in MYCOVIRO - CH 59 Book Deck (50):
1

Except for disease caused by ___, humans are resistant to infections caused by fungi.

dimorphic fungi

2

Classification of Fungi based on Appearance of Colonies
moist, creamy, opaque, pasty colonies

yeasts

3

Classification of Fungi based on Appearance of Colonies
– fluffy, cottony, woolly or powdery colonies

Mold or Filamentous fungi

4

– exhibit either a yeast and filamentous forms

Dimorphic fungi

5

– when dimorphism is temperature dependent

Thermally dimorphic fungi

6

Thermally dimorphic fungi produce a mold form at what temp

25C – 30C

7

Thermally dimorphic fungi produce yeast form at what C

35-37C

8

– fungi that have more than one independent form or spore stage; not temperature dependent

Polymorphic fungi

9

4 Phyla
-includes organisms that produce sparsely septate hyphae

Zygomycota

10

Zygomycota
-asexual reproduction by ?
-sexual reproduction by ?

sporangiospores
zygospores

11

what phyla
ex. are Rhizopus, Mucor, Rhizomucor, Absidia, Cunninghamella

Zygomycota

12

what phyla
filamentous
produce true septate hyphae

ascomycota

13

asexual repro of ascomycota

conidia formation

14

sexual repro

ascopore

15

2 forms of ascomycota

teleomorph and anamorph

16

what phyla is ubiquitous in nature

ascomycota

17

– fungi that have different asexual forms of the same fungus

*Synanomorphs

18

sexual reproduction of basidiomycota

basidiopores

19

plant pathogens; rarely cause disease in humans

basidiomycota

20

-includes the smuts, rusts mushrooms, Cryptococcus neoformans complex

basidiomycota

21

– teleomorphic form of Cryptococcus neoformans

*Filobasidiella neoformans

22

– lack a sexual reproductive cycle
-asexual reproduction through conidia

4. Deuteromycota

23

also called fungi imperfecti

Deuteromycota

24

dimorphic fungus; has not been assigned to any phylum so it is placed in the order Incertae sedis

Blastomyces dermatitidis –

25

in which order is Blastomyces dermatitidis assigned

Incertae sedis

26

classification of fungi based on type of infection

superficial or cutaneous
deep or systemic
subcutaneous
opportunistic

27

-involve the hair, skin, or nails without direct invasion of deeper tissue

superficial or cutaneous

28

mycoses which all infect keratinized tissues

superficial or cutaneous

29

– causes infection to the subcutaneous tissue only (no dissemination to other sites)

Subcutaneous mycoses

30

examples are Chronomoblastomycosis, mycetoma, phaeohyphomycotic cysts

Subcutaneous mycoses

31

most common opportunistic mycoses

• Aspergillus
• Candida
• Cryptococcus

32

– may cause deeply invasive phaeohyphomycoses (produce brown-pigmented structures)

*Dematiaceous fungi

33

type of pathogen which occurs in immunocompromised host
-not always as virulent, may lead to subclinical disease

primary

34

type of pathogen, almost any fungus present in the environment

opportunistic

35

virulence factors (4)

• Size (should be small to reach alveoli)
• Ability to grow at 37C and neutral pH
• Conversion of dimorphic fungi from mycelial form to corresponding yeast or spherule form in the host
• Toxin production

36

Many fungi have a primary focus in the lungs, thus ___ are almost always included among specimens selected for culture.

respiratory secretions

37

antifungal agent
-prevents overgrowth by rapidly growing molds
-should be included in at least one of the culture media

cycloheximide

38

amount of RT secretions for inoculation

0.5 ml

39

CSF must be filtered through a __ um membrane filter attached to a sterile syringe

0.45 um

40

CSF Specimen
If

1-drop aliquots

41

CSF
Media should have no ____

antibacterial or antifungal agents

42

if CSF processing is delayed, w

• keep at room temperature
• or place in a 30C incubator

43

Automated blood culture systems (3)

• BACTEC
• BacT/ALERT
• VersaTREK

44

lysis-centrifugation system
-for dimorphic fungi from blood
-optimal for H. capsulatum recovery and other filamentous fungi

ISOLATOR

45

Isolator is optimal for ____ recovery and other filamentous fungi

H. capsulatum

46

temp and incubation time for blood specime

optimal temperature: 30C and incubation time: 21 days

47


-submitted for dermatophyte culture

Hair, Skin, Nail Scrapings

48

hair, skin, nail obtained by

scraping

49

hair, skin, nail obtained by scraping using

scalpel blade or microscope slide

50

mycosel agar contains

cycloheximide and chloramphenicol

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