Nitrate Transport Paper

Question 1

What are LATS and HATS for Nitrogen uptake

Answer 1

LATS - low affinity nitrate transporter
Unregulated when there is a high abundance of nitrate in the soil

HATS - high affinity nitrate transporter
Unregulated when there is low amounts of nitrate in the soil

Symporters with H+

Question 2

How does the plant regulate between HAT and LAT expression?

Answer 2

CEP sense nitrate levels in soil -> travel from root to shoot.
CEP reception in shoot produces CEPD1/2
CEPD1/2 travel to roots and regulate transporter expression

Question 3

What amino acid substitution is involved in a phosphomimetic mutant

Answer 3

Typically Serine (S) —> Aspartate (D)

Leads to constitutive active phosphorylation state of the kinase

Question 4

What are the phosphorylation dynamics and nitrate uptake functions of the HAT, NRT2.1?

Answer 4

High N - phosphorylation of Ser501 functions = inactivation of the protein (no transport)
Low N – dephosphorylation of Ser501 = activation of the protein (high affinity transport)