NUCLEIC ACID RESOLUTION AND DETECTION

Question 1

First person to develop an electrophoresis apparatus.

Answer 1

Arne Tiselius

Question 2

First used starch as a matrix (15g starch/100 mL DW; 15%) to separate serum proteins.

Answer 2

Oliver Smithies (1950)

Question 3

Described 20 serum proteins using 2DGE (1D - paper; 2D - starch)

Answer 3

Smithies and Poulik (1956)

Question 4

The measure of the electric charge of a particle relative to its mass

Answer 4

Charge-to-mass ratio

Question 5

Migration depends on

Answer 5

Size of DNA
Compactness of matrix
Net charge of molecule
Shape of molecule
Electric field strength or voltage
Pore size of gel
Temperature

Question 6

Migrates the molecule in one direction

Answer 6

Gel (Gel systems)

Question 7

GEL SYSTEMS:
A polysaccharide polymer extracted from seaweed (red algae; Gelidium spp., Gracilaria spp., Gelidiella spp.)

Answer 7

Agarose Gels

Question 8

Repeating unit of Agarose gels is

Answer 8

Agarobiose

Question 9

Realignment angle of Pulsed-Field Gel Electrophoresis

Answer 9

120 degrees

Question 10

Pulsed of current applied to the gel in alternating dimensions enhance migration

Answer 10

Pulsed-Field Gel Electrophoresis