Nucleic Acids Flashcards Preview

Fundamentals of Molecular Medicine > Nucleic Acids > Flashcards

Flashcards in Nucleic Acids Deck (34):
1

 

 

 

Gout and Orotic Aciduria

2

 

 

 

What is Gout?

3

Which one of the following is not a function of a purine or pyrimidine?

Energy Source 

Precursor for Nucleic Acid Biosynthesis 

Precursor for cofactor synthesis

Second messengers 

Allosteric regulators 

Oxygen Transport 

 

 

Energy Source — ATP is an energy source

Precursor for Nucleic Acid Biosynthesis 

Precursor for cofactor synthesis— NAD

Second messengers — cAMP, AMP

Allosteric regulators — AMP

Oxygen Transport 

4

The nucleoside formed when hypoxanthine is the base is:

Zanthine

Hypoxanthosine

Adenosine

Inosine

 

 

 

Inosine

5

 

 

 

Thymine

 

 

Thiamine — comes from dUMP and N5, N10 MethylTHF

6

 

 

 

Purine Synthesis 

2 Different Pathways

 

 

 

Dietary intake of intact Purines is Zero. They are not contributing to our salvage pathway. All dietary Purines are converted to Uric Acid

7

 

 

 

 

8

The formyl carbon in N10 formyl THF cab be derived from which one of the following?

Pyruvate

Glycine 

Leucine

Lysine

Glutamate

 

 

 

Glycine — Glycine Cleavage Enzyme — Goes to N5, N10 which can go in two steps to N10 Formyl THF

9

 

 

 

Tetrahydrofolate

10

The ribose for purine synthesis can be derived from all of the following EXCEPT for which one?

Glucose

Fructose

Lactate

Succinate

Hydroxybutarate

 

 

 

Hydroxybutarate — KB cannot be used to make sugars. All others are glycogenic.

11

 

 

 

Synthesis of PRPP 

12

 

 

 

Committed Step of Purine Biosynthesis

13

Which one of the following could lead to an increase in PRPP Levels?

Type I Diabetes

Von Gierke Disease 

McArdle Disease

MCAD Deficiency

G6PDH Deficiency 

Type I Diabetes

Von Gierke Disease — G6Phosphatase deficiency— goes through HMP shunt — Gout is a symptom because of elevation G6P

McArdle Disease

MCAD Deficiency

G6PDH Deficiency — G6P doesn’t accumulate as much as G6P. 

14

 

 

 

Outline of Purine Ring Biosythesis 

15

 

 

 

What you should know about Purine DeNovo Pathway 

16

 

 

 

Biosynthesis of AMP from IMP

17

 

 

 

Biosynthesis of GMP from IMP

18

 

 

 

Conversion of AMP, GMP to more phosphorylated forms

19

 

 

 

Regulation of DeNovo Purine Synthesis 

20

 

 

 

Purine Synthesis and Gout

21

 

 

 

Purine Salvage

22

 

 

 

Purine Nucleoside Phosphorylase Deficiency 

23

 

 

 

HGPRT Deficiency 

24

 

 

 

Adenosine Deaminase Deficiency 

25

 

 

 

Purine Nucleotide Cycle 

26

What other enzyme reaction aids in buffering?

Pyruvate Dehydrogenase

Glutamine Synthetase

Glutaminase

Pyruvate Carboxylase

Alcohol dehydrogenase

Aldehyde Dehydrogenase

 

 

 

Glutaminase

27

 

 

 

Serine Derivation and Making THF

28

 

 

 

Formate degredation can be used to make...

29

 

 

 

Histidine Degredation can be used to make...

30

 

 

 

Glycine can make...

31

 

 

 

dUMP to TMP uses?

32

 

 

 

Conversion of Glycine to Serine can be used to make...

33

 

 

 

Homocysteine to Methionine uses...

34

 

 

 

Purine Biosynthesis requires...

Decks in Fundamentals of Molecular Medicine Class (77):