Flashcards in Nucleotide Metabolism Deck (32):
what are the functions of nucleotides?
-building blocks of DNA and RNA
-carriers for activated intermediates
- components of CoA, FAD, NAD+ and NADP+
what is the structure of nucleotides?
- phosphate groups
what is hypoxanthine?
base found in IMP - an intermediate in purine synthesis and degradation
what is xanthine?
found in xanthosine MP - intermediate in purine synthesis and degradation
what makes up a nucleoside?
N base + pentose sugar
What charge do the phosphate groups have?
where does de novo purine synthesis take place?
cytoplasm of cell
what are the contributors to purine synthesis?
-2 C from THF
- 1 CO2
what is the RLE and cmootied enzyme for purine synthesis?
glutamine phosphoribosyl pyrophosphate amidotransferase
what 2 enzymes do purine salvage require?
hypoxnthine-guanine phosphoribosyltransferase (HGPRT) and
adenine phosphoribosyltransferase (ARPT)
what are most purine degraded into?
what 2 drugs treat gout?
what activates glutamine PRPP amidotransferase?
glutamine and PRPP
what inhibits glutamine PRPP amidotransferase?
AMP, GMP, IMP
what do you need for all purine synthesis?
what is the 1st purine made?
how much of nucleotides are degraded?
10% -- 90% are salvaged/recycled
what are the contributors for pyrimidine synthesis?
- aspartic acid
what is the RLE in pyrimidine synthesis?
where is CPSII?
where does the N come from in CPSII and CPSI?
CPSI - ammonia
CPSII - glutamine
what activates CPSII?
what inhibits CPSII?
what is the only enzyme that converts ribo--> deoxyribo ?
what does ribonucleotide reductase only work on?
how is orotic aciduria treated?
what are the inhibitors of ribonucleotide reductase?
what are the 3 drugs we talked about that are used in chemotherapy?
what is the only pyrimidine that requires THF?
what is the target of methotrexate?
what is the target of hydroxyurea?