Nucleotide Metabolism (complete) Flashcards Preview

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Flashcards in Nucleotide Metabolism (complete) Deck (85)
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1

Which bases are purines

Adening
Guanosine

2

which bases are pyrimidines

Cytosine
thymine
Uracil

3

what is the structure of the pyrimidines

an individual 6 membered ring

4

what is the structure of purines

a 2 membered ring with an attached 5 membered ring

5

how do you differentiate between Adenine and Guanosine

Adenine has a NH2
Guanine has a carbonyl

6

how do you differentiate between Cytosine, thymine, and uracil

Cytosine has NH2
thymine has a methyl group
Uracil has neither

7

1. Nucleotides are only involved in RNA and DNA synthesis
a. True
b. False

False

8

3. A nucleoside is
a. A nucleobase
b. A nucleobase and a ribose sugar
c. A nucleobase, a ribose sugar and a phosphate group
d. A nucleobase, a ribose sugar, a phosphate group and acetyl CoA

B

9

4. Purine biosynthesis regulated by:
a. AMP,
b. ADP
c. IMP
d. GMP
e. All of the above

E

10

6. Which is the final step in the pathway for pyrimidine biosynthesis
a. UMP
b. CMP
c. TMP
d. TTP

B

11

7. 5-fluorouracil is an inhibitor of
a. purine bio synthesis
b. pyrimidine biosynthesis
c. thymidine synthesis
d. Adenosine synthesis

C

12

8. Another name for an anti-metabolite is:
a. Anti-toxin
b. Anti-freeze
c. Inhibitor
d. Allosteric modifier

Inhibitor

13

9. Ribonucleotide Reductase activates the tyrosine radical with
a. Iron cofactor
b. Copper cofactor
c. NAD cofactor
d. Folate cofactor

A

14

What are the functions of nucleotides

Energy for metabolism (ATP)
Enzyme cofactors (NAD+)
Signal transduction (cAMP)

15

what are the functions of nucelic acids

Storage of genetic info (DNA)
transmission of genetic info (mRNA)
processing genetic info (ribozymes)
protein synthesis (tRNA and rRNA)

16

What is a nucleobase

nitrogenous base (A, T, U, C, G)

17

What is a nucleoside

nitrogenous base and a pentose (the sugar)

18

What is a nucleotide

nitrogenous base, pentose, and phosphate (1, 2, or 3)

19

in de novo biosynthesis of purines are the bases and ribose made seperately and then put together

no, the base is synthesized while attached to the pentose

20

what is the pentose used in DNA and RNA

ribose

21

what amino acid provides most amino groups for newly made nucleotides

Gln (glutamine)

22

what is the precursor for purines

glycine (gly)

23

what is the precursor for pyrimidines

Asp (aspartic acid)

24

What things contribute to the formation of a purine

aspartate (N)
CO2 (C)
formate (2-Cs)
glycine (C=C-N)
glutamine (2-Ns)

ACFGG

25

what does de novo biosynthesis of purines begin with

PRPP

26

in the de novo biosynthesis of purines what does PRPP interact with

Gln (adds an N)

27

after PRPP has reacted with Gln in de novo biosynthesis what does that molecule interact with

Gly (adds C=C--N)

28

What is the first intermediate in de novo biosynthesis of purines that has a full purine ring

inosinate (IMP)

29

what things can by synthesized from IMP

Adenine and Guanine

30

How does inosinate (IMP) get converted to Adenylate (AMP)

1. inosinate + aspartate and GTP = adenylsuccinate
2. Adenylsuccinate + Fumerate = adenylate