Para Lab - Quiz 1 Flashcards Preview

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Flashcards in Para Lab - Quiz 1 Deck (49):
1

– most common specimen

stool sx

2

amount of stool collected (Formed)

2-5 g
about size of walnut or thumb

3

must be labelled with

Name
Age
Date and time of collection

4

how many stool specimens are collected in different days to increase chances of detecting the parasite

3

5

stool sx must be accompanied with

a lab request

6

brown color of stool due to

stercobilin

7

• Gross abnormalities – presence of abnormal structures such as

Mucus
o Pus
o Proglottids (Segment of a tapeworm)
o Blood

8

uses iodine and saline wet preparations for screening purposes

DFS

9

for detection of helminths (parasitic worms) & protozoans

DFS

10

For detection of trophozoite and cyst stages

DFS

11

Disadv of DFS

not sensitive because of very small amount of Sx used

12

can hide fecal organisms

Fecal debris

13

ADV of conc technique

-advantage: increases the chances of parasite detection because a larger amount of stool is used

14

DISADV of conc technique

-disadvantage: cannot preserve or detect trophozoites because they are easily destroyed

15

2 methods of conc technique

flotation
sedimentation

16

used for small eggs or cysts that tend to float

flotation

17

-principle of flotation

all eggs and cysts will float

18

flotation not suitable for protozoans but useful for demonstration of helminthic eggs

brine flotation

19

-Sp Gr of brine solution

= 1.20

20

measures specific gravity

Refractometer

21

Advantages of brine

Inexpensive
• Applicable in field work

22

Disadvantages of brine

Badly shrunken morphology of some eggs and cysts
Eggs of Clonorchis and Opistorchis do not float

23

useful for: recovery of protozoan cysts and helminth eggs

ZnSO4 flotation

24

sp gr in zinc sulfate flotation

Sp Gr = 1.18 – 1.20

25

ZnSO4 flotation NOT useful for

• Large trematode eggs
• Some tapeworm (cestode) eggs
• Ascaris lumbricoides unfertilized eggs

26

useful for detection of Cryptosporidium

Sheather’s Sugar Flotation

27

Solution added in Sheather’s Sugar Flotation

sucrose-phenol solution

28

used for heavy or large eggs or cysts

sediments

29

FECT meaning

Formalin Ether Concentration Technique

30

FECT Components
Components:
– preservative/fixative

10% formalin

31

FECT Components
removes fecal fats
-difficult and dangerous to handle, highly volatile

ether

32

FECT Components
to strain fecal suspension

gauze

33

FECT Components
optional only (done for better visualization

Lugol's iodine

34

FECT Components
removes fecal debris

conical tube

35

a substitute for 10% formalin but may destroy Blastocystis hominis

distilled water

36

susbtitute for ether

ethyl acetate

37

4 Layers that will be Formed in sedimentation (from top to bottom)

1. Ether
2. Debris
3. Formalin
4. sediment

38

1. Used for quantitating helminth eggs
2. Used to determine intensity of infection (↑ eggs, ↑ intensity of infection)
-results expressed as EPG or eggs per gram

kato-katz technique

39

another name of kato-katz

cellophane technique

40

KK components
pleasing to the eyes; soaked overnight in glycerol

Green cellophane

41

sub for green cellophane

substitute: 3% Aqueous Malachite Green or 3% Methylene Blue Stain + transparent cellophane

42

KK components
acts as a clearing agent to be able to view the Sx clearer

50% glycerol –

43

KK components
calibrated

template

44

template thickness and diameter

thick: 1.5 mm
dia: 6mm

45

KK components
removes large fecal debris for easy viewing

Wire mesh

46

KK components
– used to evenly distribute/spread stool
-

Rubber stopper/cork

47

sub for rubber stopper or cork

substitute: mineral bottle cover

48

KK components
used in pressing the wire mesh and scraping of the sieved stool

Popsicle stick

49

hastens the clearing process

Heat (40C) or direct sunlight

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