part8 Flashcards
(24 cards)
- Name the phase of the cell-cycle when the chromosomes are correctly aligned on the mitotic spindle.
M-phase
. What is the function of APC?
Triggers transition from metaphase and anaphase-ubiquitin ligase that targets securing for degradation, and targets securin for degradation leading to release of separase that cleaves the cohesion complex
What produces the force to form the cleavage furrow during cytokinesis?
Actin-Myosin II Contractile Bundles
Which of these amino acid sequences is a putative ER retrieval signal?
a) –KKXX
b) –NPXY
c) –YXXØ
d) –NXT-
-KKXX
Vesicles that carry cargo from the ER to the cis Golgi have which type of coat?
COPII
The coupling of which protein to the epidermal growth factor receptor acts as a signal for sorting at the late endosome/ multivesicular body?
Rab
Why do electron microscopy specimens need to be presented on very thin sections?
So that electrons can pass through the sample
What does FRET stands for?
Fluorescence (or Forster) Resonance Electron Transfer
What is Danio rerio (the Zebrafish) used for as a model? Give two or more examples.
Used to study Internal Development (As Visible/Transparent)
Used to study eye, cardiovascular and neurological studies
Phospholipid bilayer membranes form spontaneously because of a reduction in which thermodynamic property?
Entropy
Which one of these molecules is found embedded in eukaryotic cellular membranes:
a) phosphatidylsphingosine
b) phosphatidylceramide
c) phosphatidylinosine
d) phosphatidylethanolamine
Phosphatyidylethanolamine
How thick is a typical cell membrane? Units must be clearly stated.
6nm
Which protein kinase is activated by AMP?
mTOR
Name two signalling molecules which are negative regulators of cell size and growth.
PTEN, TSC2
What is the main application of yeast two-hybrid screening?
Protein-Protein Interactions
. Which pair of organisms shared a common ancestor most recently? (circle one answer only)
a) banana / human b) Euglena / baker’s yeast c) banana / baker’s yeast d) human / baker’s yeast
Euglena/Baker’s Yeast
For import of proteins into mitochondria which statement is true? Circle one answer only.
a) Proteins fold before passing through the mitochondrial outer membrane
b) No energy is required for protein uptake into mitochondria
c) The machine that inserts proteins into the outer membrane after
they have been translocated into the intermembrane space has
recognisable bacterial homologues
d) The machine that inserts proteins into the outer membrane before
they have been translocated into the intermembrane space has
recognisable bacterial homologues
The machine that inserts proteins into the outer membrane after
they have been translocated into the intermembrane space has
recognisable bacterial homologues
Which enzyme is found in peroxisomes:
a) catalase b) ATP synthase c) ribonuclease d) acid phosphatase
Catalase
What are the three defining characteristics of somatic stem cells?
Self Renewal
High Proliferation Potential
Multipotent (Not terminally differentiated)
What does GM-CSF stand for?
Granulocyte Macrophage-Colony Stimulating Factor
Name three areas of the body where bone marrow is found in adults?
Pelvis, Sternum, Cranium (and Long Bones such as the Femur)
Which is the major mitochondrial regulator of the permeability transition pore?
Ca2+ levels
What is the immediate consequence of a fall in mitochondrial membrane potential?
No Oxidative ATP generation
What are the enzymes called that dismantle the cell during apoptosis?
Caspases
