Practical 1 - Aseptic technique and handling bacteria Flashcards Preview

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Flashcards in Practical 1 - Aseptic technique and handling bacteria Deck (16):
1

What is sub-culturing?

Sub-culturing is the aseptic transfer of microorganisms from a culture to a fresh medium

2

What is aseptic technique?

Aseptic technique refers to a procedure that is performed under sterile conditions.

3

What does sterile mean?

free from bacteria or other living microorganisms

4

How many sub-culturing procedures are there?

four

5

List the four sub-culturing procedures.

Solid to solid
Solid to liquid
Liquid to solid
Liquid to Liquid

6

What is the solid to solid sub-culturing procedure?

The transfer of a microbe from an agar slope or plate culture to an agar plate

7

What is the solid to liquid sub-culturing procedure?

The transfer of microbes from an agar slope or plate culture to a broth

8

What is the liquid to solid sub-culturing procedure?

The transfer of microbes from a broth culture to an agar slope or plate

9

What is the liquid to liquid sub-culturing procedure?

The transfer of microbes from a broth culture to a broth

10

The use of aseptic technique minimises the risk of...

contamination of cultures as well as cultures escaping to the environment

11

How must the wire/inoculating loop be sterilised?

By flame sterilisation

12

Why must the inoculating loop be passed through a blue flame until red hot?

To make sure that any contaminating bacterial spores are destroyed

13

How often should you flame the loop?

Before and after the transfer of microbes

14

What is a pure culture?

Cultures that contain only one type of microorganism

15

The streak technique allows you to

produce discrete colonies

16

What is a colony?

A population of cells arising from a single cell or spore or from a group of attached cells