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Flashcards in Proteins Deck (54)
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1

What are the 2 properties of a secondary protein structure?

strength & flexibility

2

Differentiate tertiary fr quaternary structure of protein

tertiary - overall shape or conformation
of the protein molecule

quaternary - shape from interaction of more than
one protein molecule

3

Differentiate conjugated from simple proteins

conjugated: consists of a protein & prosthetic group + prosthetic group defines the characteristics of the protein

simple: contain peptide chains composed of only AA

4

Differentiate glomerular fr fibrous simple protein

Globular – symmetrical & soluble in water
- transporters, enzymes & messengers
Fibrous – asymmetrical, inert, with hydrophobic R groups

5

Classify the ff proteins accdg to their structure(be specific):
A. ferritin
B. chromatin
C. collagen
D. immunoglobulin

A. conjugated - metalloprotein
B. conjugated - nucleoprotein
C. simple - fibrous
D. simple - globular

6

Classify the ff proteins accdg to their structure(be specific):
A. albumin
B. troponin
C. flavoprotein
D. haptoglobin

A. simple - globular
B. simple - fibrous
C. conjugated - metalloprotein
D. conjugated - glycoprotein

7

Which is true?
A. Isoelectric point: number of positive = negative charge groups.
B. If a protein is placed in solution that has a pH > pI , it is (+) charged
C. If a protein is placed in solution that has a pH

A

baliktad ang B at C
D - mali, it has both positive and negative charges

8

Which is false?
A. at low pH, amino and carboxyl groups are protonated
B. at high pH, amino group is also deprotonated
C. most proteins are positively charged at physiological pH

C

negative dapat

9

Enumerate 5 properties of protein used for sep, identify & assay

Differential solubility
Adsorption on finely div inert matl
Molecular size
Electrical charge
Specific binding

10

Which is false?
A. Endogenous protein synthesis is provided by dietary intake of protein
B. Body's amino acid pool - source for plasma, intracellular protein
C. Urea is synthesized from ammonium ions c/o deamination
D. There is both nitrogen pool & storage for amino acids in the body

D

nitrogen pool only

11

Two pathways for protein catab - differentiate and enumerate

Lysosomal - intracellular and extracellular protein only

Cytosolic - intracellular protein only

12

Transaminations remove nitrogen from free amino acids; this produces two substances. Enumerate & mention their fates

Ammonia - converted to urea and excreted in urine
Ketoacids

13

Amino acid pool/nitrogen pool interacts with all except
A. Heme & CO2
B. Glucose
C. Urea
D. Vitamins

D

14

False about transthyretin
A. Transports retinol & protein for T3
B. Has a long half-life (2mos)
C. Marker for poor nutri status
D. Inc with steroid use

B

2 days lang

15

False about albumin
A.maintains fluid balance in tissue
B.buffers pH
C.binds blood substances
D.positive Acute phase protein

D
Negative dapat

16

Glycosylated Hb - glucose trend over __
Glycated albumin - glucose trend over __

(Identify number of mos)

Hb - 3
Alb - 1

17

Not a cause of decreased albumin
A.diarrhea
B.hypothyroidism
C.hemoconcentration
D.polydipsia

C

18

False about alpha-1 antitrypsin
A.most impt agonist for leukocyte elastase
B.major constituent for alpha-1 globulin
C.elevated by estrogen
D.NOTA

A
Inhibitor dapat

19

Which method is used to determine very low conc of albumin?
A.bromocresol green/purple dye
B.use of serum
C.immunochemical method

C

20

Alpha-1 fetoprotein : protects fetus fr immuno attack of mom. Seen in all except
A.spina bifida
B.defects for renal tubule
C.anencephaly
D.general fetal distress

B - neural tube/abdominal defect

21

Haptoglobin helps detect hemolytic anemia by seeing decrease in all except
A.heptoglobulin
B.reticulocytes
C.hematocrit
D.hemoglobin

B

22

Which is not used to test alpha-1 fetoprotein level
A.RIA
B.EIA
C.RID

C- for haptoglobin

23

Plays role @ inflammation/carcinogenesis
A. Inter-alpha-trypsin inhibitor
B. Gc-globulin
C. Alpha1-acid glycoprotein
D. Ceruloplasmin

A

24

Transports endotoxin, carries vitD, high @ preg & low @ liver damage. Measured via immunonephelometry
A. Inter-alpha-trypsin inhibitor
B. Gc-globulin
C. Alpha1-acid glycoprotein
D. Ceruloplasmin

B

25

Positive APP that is soluble in water. It may reduce bioavailability of some drugs
A. Inter-alpha-trypsin inhibitor
B. Gc-globulin
C. Alpha1-acid glycoprotein
D. Ceruloplasmin

C

26

Used to diagnose Wilson's disease and Kayser-Fleischer in cornea
A. Inter-alpha-trypsin inhibitor
B. Gc-globulin
C. Alpha1-acid glycoprotein
D. Ceruloplasmin

D

27

Which is not an Acute phase reactor in humans?
A. Inter-alpha-trypsin inhibitor
B. Gc-globulin
C. Alpha1-acid glycoprotein
D. Alpha-2 macroglobulin

D

28

False about siderophilin
A.levels determined to determine anemia cause + iron metab&capacity
B.major comp of B-globulin fraction
C.high levels in malignancy
D.reversible bonds with ferric ion

C
Low dapat. Siderophilin is just transferrin

29

Excess iron dep in tissues, leading to bronze skin, diabetes mellitus, cirrhosis ETC

Identify

Hemachromatosis

30

Transferrin saturation - formula

Iron conc / TIBC