pyruvate dehydrogenase Flashcards
what does pyruvate dehydrogenase do
catalyzes the oxidative decarboxylation of pyruvate to form acetyl CoA and NADH in the mitochondrial matrix
what family does pyruvate dehydrogenase belong to
a family of enzymes, all sharing the same mechanism and cofactor requirements
what are the names of the 3 catalytic activities that make up multienzyme complexes
E1= pyruvate dehydrogenase
E2= dihydrolipoyl transacetylase
E3= dihydrolipoamide dehydrogenase
what does E1/ pyruvate dehydrogenase do
oxidative decarboxylation of pyruvate with production of CO2
what does E2 / dihydrolipoyl transacetylase do
acetyl group donated to CoA to form acetyl CoA
what does E3 / dihydrolipomide dehydrogenase do
NADH formed from reduction of NAD+
how many coenzymes are required for the activity of the complex to happen
5
what are the 5 coenzymes required for the activity of the complex
1) Thiamine pyrophosphate (TTP) bound to E1 (vit B1)
2) lipoamide bound to E2 (lipoic acid)
3) FAD bound to E3 (riboflavin)
4) coenzyme A required for E2 (pantothenic acid)
5) NAD+ required for E3 (nicotinamide)
per molecule of glucose entering glycolysis what are the products
2 CO2
2 Acetyl CoA
2 NADH + H+
where does the 2 CO2 produced go
waste
where does the 2 Acetyl CoA produced go
TCA cycle
where does the 2 NADH + H+ produced go
ETC
what inhibits activity for the negative allosteric modulation
1) acetyl CoA via dihydrolipoyl transacetylase (E2)
2) NADH via dihydrolipoyl dehydrogenase (E3)
