Qs 31-40

Question 1

To determine a titer of antibodies is used a _____ dilution of serum.

Answer 1

two fold.

Question 2

Double radial immunodiffusion with antibodies against ag A and ag B produced theese precipitation lines:

Answer 2

Ag B in well 3
Ag B in well 2
Ag A in well 1
Ag B in well 6

Question 3

To create a calibration curve in immunodiffusion test, we need to know?

Answer 3

Antigen concentration and diameter of precipitation rings.

Question 4

Agarose gel is prepared by:

Answer 4

boiling of agarose suspension.

Question 5

The speed of the movement of molecules during electrophoresis is dependent on:

Answer 5

Molecular mass.

Question 6

In an indirect ELISA for testing of antibodies, the intensity of color after adding of the substrate in the final step, is directly proportional to the concentration of:

Answer 6

antibodies in patient serum.

Question 7

A negative reaction in a tube agglutination test is:

Answer 7

White sediment on the bottom and turbid supernatant.

Question 8

Which immunoelectrophoresis is for quantitative determination of antigen?

Answer 8

Rocket immunoelectrophoresis.

Question 9

Result of immunoprecipitation in the gel is visible after at least?

Answer 9

24h incubation at room temp

Question 10

Antibody is added to agarose used for:

Answer 10

Single radial immunodiffusion.