Ram semen collection and artificial insemination Flashcards Preview

ANSC3102 - Prac 1 > Ram semen collection and artificial insemination > Flashcards

Flashcards in Ram semen collection and artificial insemination Deck (21)
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1
Q

Method of semen collection:: from genital tract of female

A
  • By sponge or ladel

Disadvantages: semen can be contaminated with mucus of female, urine, and genital diseases can be spread.

2
Q

Method of semen collection: Massage method

A

Manual or digital stimulation

Disadvantages: in the bull: semen can be contaminated with urine and bacteria.

3
Q

Method of semen collection: Electrical ejaculation

A

Original type had two electrodes, rectal and lumbar.
Recent apparatus has bipolar electrodes.
Finger electrode.

Disadvantages: 
causes discomfort to the animal 
the animal must be restrained
semen can be contaminated with urine 
More likely to get immature sperm
frequent collections within one day cannot be made 

Advantages:
useful when the animal lacks libido or refuses to serve into A.V.
when the animal
is injured, unfit or old and is incapable of serving
it is particularly useful for testing large numbers of rams for semen quality
after vasectomy
for sale, etc.

4
Q

Methods of semen collection: Artificial vagina

A
Advantages: 
it is a natural way of semen collection
semen of good quality can be obtained
no difficulty in practical application
it is a cheap and quick method
frequent collections within
one day can be made. 

Disadvantages:
the male should be trained to serve into the A.V. Rams have different
temperaments.

5
Q

Cervical A.I

A

Non invasive

Short, straight forward procedure

6
Q

Insemination dose for vaginal A.I

A

Largest dose

400 mil

7
Q

Insemination dose for cervical A.I.

A

100 mil

8
Q

Insemination dose for intrauterine A.I

A

25 mil

9
Q

How much sperm in one ejaculate?

A

4-5 billion

One ejaculate can inseminate 40 ewes (A.I.)

10
Q

Synchronising ewes for A.I.

A

Intra vagina progesterone sponge or seeder
Leave sponge in for 12 days
PMSG injection given at the time of sponge removal - helps stimulate ovulation
Teaser rams can also be introduced into the paddock at this time - stimulates oestrus and ovulation

Estradiol peaks
Triggers LH surge
Ovulation around 24 hours after LH surge

Should inseminate 48hours after sponge removal (if using fresh semen)

11
Q

LH surge triggers oocyte development

A

Resumption of meiosis

Loss of polar body

12
Q

How long does it take for ovulation to occur after sponge removal?

A

60 hours

13
Q

How long does it take for a ewe to come into oestrous after sponge removal?

A

38- 48 hours

Lasts for 24 - 36 hours

14
Q

When is PMSG injected into ewes?

A

At the time of sponge removal - helps stimulate ovulation

15
Q

How much PMSG is injected into ewes?

A

400 - 500 units

16
Q

Fecundin immunisation

A

Used on un-synchronised ewes

Increases the twinning rate

Requires 2 injections

  1. 5 weeks prior to mating
  2. Booster injection given 3 weeks after first injection
17
Q

Regulin

A

Subcutaneous implant used to advance the breeding season and also increases twinning

Works best in conjunction with the ram effect

18
Q

When is laproscopic intrauterine insemination used?

A

For frozen semen

or insemination of superovulated ewes

19
Q

laporoscopy methods

A

Deprive ewes of food and water for 12 hours
Cradle is used to restrain the ewe
Clip the ewe
Local anaesthetic is applied in 2 regions, a few cm either side of the midline
Skin is sterilised with ethanol
Ewe is presented for insemination in a position of dorsal recumbancy at an angle of 40 degrees from the horizontal
Abdomen is inflated with CO2

20
Q

Laproscopic A.I fertilisation rates in superovulated ewes

A

90%

21
Q

Laproscopic A.I with forzen semen - % of lambs expected

A

65 - 70%