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Packet Info > RANDOM > Flashcards

RANDOM Flashcards

1
Q

Advantage of using dry heat sterilization over autoclaving?

A

No dulling, it keeps instruments sharp

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2
Q

Best method to sterilize instruments without corrosion?

A

Dry Heat

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3
Q

Ethylene oxide for sterilization is good for?

A

Heat Labile Tools

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4
Q

If unable to use heat, what method should be used to sterilize instruments?

A

Ethylene Oxide

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5
Q

What’s a way to measure human basal energy?

A

Temperature

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6
Q

What enzymes are not involved in making cDNA?

A

Telomerase

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7
Q

Reverse Transcriptase & RNA-dependent

A

DNA-polymerase are used

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8
Q

DNA fingerprinting?

A

Restriction site

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9
Q

Which of the following types of blotting can be used to ID DNA restriction fragments?

A

Southern blotting

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10
Q

Restriction endonuclease are used to cut?

A

DNA

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11
Q 
Northern = RNA
Southern = DNA
Western = ?
A

Western= Proteins

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12
Q

Know the steps of PCR: amplify & make copies of DNA

A
  1. Initialization: DNA polymerase that need heat activation
  2. Denature: heat reaction for 20-30 sec so DNA melts/ disrupt H-bonds to yield ssDNA
  3. Annealing: reaction T is lowered for hybridization of primer to strain
  4. Extension: DNA polymerase added to synthesize new DNA complement, x2 DNA
  5. Final Elongation: ensure all DNA strands have elongated
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13
Q

When does ph=pKa

A

Isoelectric point (isoeletric point is the pH such that no migration occurs during electrophoresis)

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14
Q

Mechanism of Fluoride

A

Enolase Inhibitor (which inhibits glycolysis)

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15
Q

Regulation of F

A

bone/urine

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16
Q

What innervates the erector spinae muscles?

A

Dorsal Branches of the Spinal N

17
Q

Which lymph nodes are along the external vein?

A

Deep cervical lymph nodes

18
Q

Which structure has both afferent & efferent vessels?

A

Lymph Nodes

19
Q

Tetrodotoxin (pufferfish) is a channel blocker for voltage-gated Na+ channels

A

no action potential

Packet Info (34 decks)

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