Revision Lect 1,2,3

Question 1

Microbes for human wellfare

Answer 1

Sewage
Bioremediation
Recycling elements
Biotechnology
Pesticides

Question 2

Structures external to cell wall

Answer 2

Glycocalyx sugar coat made of polysaccharides
Organised and firm capsule and biofilms for bacterial aggregation
Loose and unorganised, slime layer

Flagella, long filamentous appendages that propel bacteria

Question 3

Flagella

Answer 3

0= atrichious
1= monotrichiois
2= amphitrichiius
Alot from 1 side= cephalotrichous
Alot from 2 sides= lophotrichous
Alot=perritrichous

Question 4

Defective cell wall bacteria

Answer 4

Spheroplast and protoplast

Question 5

Membrane

Answer 5

Selective permeability and catalysing reactions
Destructed by alcohol disrupts phospholipids causing leakage

Question 6

Nucleoid

Answer 6

Single circular double strand

Question 7

Plasmid

Answer 7

Additional
Transferred
Replicates independently or dependently
Circular double strand of DNA
Antibiotic resistance
GMOs

Question 8

Ribosome

Answer 8

30S and 50S
30. Stretomycin
50. Chloramphenicol
Protein synthesis
Granular appearance

Question 9

Inclusions

Answer 9

Volutin phosphate
Carboxysome 1,5 biphosphate carboxylase, ribulose enzyme
Lipid lipid
Gas
Polysaccharide for starch
Magnetsomes Fe3O4

Question 10

Endospore

Answer 10

Replication
Isolation
Spore septum
Peptidoglycan forespore
Protein
Cell degradation

Question 11

Total count

Answer 11

Membrane
Chamber
Electronic
Flow cytometry

Question 12

Viable

Answer 12

Plate(pour spread)
Membrane
Most probable number

Question 13

Nt=

Answer 13

N0*2^n

Question 14

CFU/ml=

Answer 14

Colonies/distillation *volume

Question 15

Plate adv

Answer 15

Inspection and morphology
Single colony

Question 16

Pour disadv

Answer 16

Heat
Beneath surface

Question 17

Spread disadv

Answer 17

Not all microbes can grow on solid

Question 18

Indirect

Answer 18

Turbidity(spectrophotometer) transmission
Dry weight (filamentous)
Metabolic activity