Serine Proteases

Question 1

Define Proteases.

Answer 1

Question 2

What is the general peptide hydrolysis reaction?

Answer 2

Question 3

Define exopeptidase and endopeptidase.

Answer 3

Question 4

Define the structure of bovine trypsin, (a serine protease).

Answer 4

Pay attention to 2 things.
1. Proteins: Asp, Hst, Ser <- catalytic triad. Very important for function.
2. Specificity pocket: the thing that makes the protease unique.

Question 5

What are the three catalytic mechanisms of Serine Protease?

Answer 5

Question 6

What are some specific functions of Serine Protease you need to know? (5)

Answer 6

Question 7

Test: explain what’s going on at each step.

Answer 7

Question 8

What happens in going to happen to the oxyanion hole? Why is it there? What does it do?

Answer 8

Question 9

What differs chemotrypsin, trypsin, and elastase from each other?

Answer 9

Specificity pocket:

Question 10

Know this: