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Flashcards in Serological Tests Deck (21):
1

ELISA

Serological

2

Neutralization

Serological

3

PCR

Molecular

4

Fluorescent Antibody Test

Serological

5

Agar gel immunodiffusion

Serological

6

Microarray

Molecular

7

Immunohistochemistry

Serological

8

Real-Time PCR

Molecular

9

Based on detection of virus genes

Molecular

10

Hemagglutination

Serological

11

Hemagglutination inhibition

Serological

12

Rely on the property of some pathogens to nonspecifically agglutinate erythrocytes

Hemagglutination and hemagglutination inhibition tests

13

Antibody tagged with an enzyme that reacts w/a substrate to produce a colored product that can be visualized in the infected cells

Immunohistochemistry

14

Antibodies labelled with a fluorescent dye and visible fluorescence appears following antigen-antibody reaction

Fluorescence antibody test (FAT)

15

Weaker signal indicates presence of antigens in sample (positive result)

Competitive ELISA

16

Antigen binds to enzyme tagged antibody and unbound antibodies are washed out before adding substrate that will change color where Ag-Ab is present

ELISA

17

Loss of infectivity through reaction of the virus with a specific antibody

Neutralization

18

Amplification of viral genome/DNA

PCR

19

Denaturation, annealing, extension/elongation

PCR

20

Advanced PCR that allows monitoring and quantification of increasing accumulation of PCR products as the reaction progresses

Real-Time PCR

21

Can detect hundreds of pathogens at once

Microarrays