SI LEC - Classes of Antibodies COPY Flashcards Preview

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Flashcards in SI LEC - Classes of Antibodies COPY Deck (75):
1

Most abundant immunoglobin,

IgG

2

% IgG

75-80% of serum Ig

3

S and MW of IgG

7S, MW=150,000

4

Longest half life (23-25 days)

IgG

5

Half life of IgG

23-25 days

6

IgG which cross the placenta

IgG1, IgG3 and IgG4

7

IgG least efficient in crossing the placenta

IgG2

8

IgG most effective complement activator

IgG3:

9

IgG High affinity for FcR on phagocytic cells, good for opsonization

IgG1 and IgG3

10

FUNCTIONS
Provide immunity to the newborn
Fix complement
Coating antigen (opsonization)
Neutralizing toxins and viruses
Participate in agglutination and precipitation

IgG

11

% IgM

5-10%

12

S and MW of IgM

19S, MW= 970,000

13

Shorter life span than IgG (about 10 days)

IgM

14

Pentameric version is secreted, Monomeric on B cell surface

IgM

15

IgM version in secretion

pentamer

16

IgM version in B cell surface

Monomer

17

5 monomeric units are held together by __ chains

J

18

More efficient than IgG in complement activation

IgM

19

Cannot cross the placenta

IgM

20

Antigen-induced conformational changes in IgM
(2)

starfish or planar
staple or crab

21

FUNCTIONS
Complement fixation
Opsonization
Agglutination
Toxin neutralization



IgM

22

*First Ig of primary immune response

IgM

23

IgG increased at what response (primary or secondary)

secondary

24

conc of IgM in primary and secondary response

SAME

25

Long lag phase
Slow exponential increase in antibody
Short lived response

Primary Immune Response

26

Anamnestic response
Short lag phase
Antibody increase is more rapid
Long lived response

Secondary Immune Response

27

% IgA in serum IgG

10-15% of serum IgG

28

S and MW of IgA

7S and MW=160 000

29

Predominant Ig in secretions

IgA

30

IgA Subclass
found in serum, mainly monomeric, polymers possible not common though

IgA1

31

IgA Subclass
found in secretions, as dimer, w/ J-chain, w/ secretory component

IgA2

32

IgA1 and IgA2: differ in __ aa (13 of which is located in the hinge region)

22

33

__ more resistant to bacterial proteinases than IgG1

IgA2

34

MW of IgA secretory component

70 000

35

IgA Structure
attached to Fc region around the hinge of the alpha chain,

Secretory component

36

IgA Structure
facilitates transport of IgA to mucosal surface,

Secretory component

37

IgA Structure
masks sites that would be susceptible to protease cleavage

Secretory component

38

not capable of C’ activation

IgA

39

FUNCTIONS of Ig
Patrol mucosal surfaces
Neutralize toxins
Helps prevent bacterial adherence to mucosa

IgA

40

extremely scarced in the serum (0.001%)

IgD

41

% IgD in serum

0.001%

42

half life of IgD

2-3 days

43

MW of IgD

MW=180,000

44

Expressed on B-cell Surface
-Play a role in B cell activation
-Regulate B cell maturation and differentiation

IgD

45

Least abundant (0.0005%)

IgE

46

% IgE

0.0005%

47

S and MW of IgE

8S, MW=190,000

48

Heat labile


IgE

49

Nuisance ab

IgE

50

Not capable of C’ fixation, agglutination, opsonization, or crossing the placenta

IgE

51

Binds mast cells and basophils thru Fc(EPSILON)R (CH3)

IgE

52

Binding causes degranulation (Histamine release)

IgE

53

IgE Binds mast cells and basophils thru __

Fc(EPSILON)R (CH3)

54

H chain genes are located on chromosome __

14

55

kappa chain genes are on chromosome _

2

56

the lambda chain genes are on chromosome __

22

57

IgG with extra constant chain

IgG and IgM

58

extra constant chain of IgG

CH2

59

extra constant chain of IgM

CH3

60

extra constant chain of IgG are sites for __

complement binding

61

Ig implicated in HDN

IgG

62

agglutination, nature of Ag

INSOLUBLE

63

precipitation, nature of Ag

SOLUBLE

64

conformation of IgM when unbound to Ab

Starfish

65

conformation of IgM when bound to Ab

Crab

66

patrol Ab

IgA

67

protects IgA from enzymes that may degrade it

secretory component

68

with polyIg receptor

IgA

69

Ab diversity
different chromosomes control different parts of Ab

Tonegawa

70

theory
1.) the lock and key concept of the fit of ab for ag 2.) the idea that an ag selected cell w/ built-in capacity to respond to it.

Ehrlich’s Side Chain Theory

71


-individual lymphocytes are genetically pre-programmed to produce one type of Ig and that a specific antigen finds or selects those particular cells capable of responding to it, causing them to proliferate

Clonal Selection Theory

72

3 Gene groups of HC genes

VH, D, J

73

How many VH

39

74

How many D

23

75

How many J

6

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