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Flashcards in Staphylococcus Deck (95):
1

what needs to be done if there is negative result in coagulase test

perform novobiocin sensitivity test

2

this is associated with TSS

F-pyogenic enterotoxin C

3

where is the primary reservoir of Staph?

nares (pwede di. sa vagina, axillae, pharynx, skin surfaces)

4

coagulase negative staph but gives a positive clumping factor test result

occasionally confused with S. aureus

S. lugdunensis

5

this staph is resistant to novobiocin

s. saprophyticus

6

result of micrococci to lysostaphin

resistant

7

staph grow easily in which agar

sheep blood agar

8

how long are specimens incubated in tube coagulase method?

4 hours

9

this enzyme acts on lipids present on the skin surface

lipase

10

also called scalded skin syndrome

Ritters disease

11

enterotoxin responsible for pseudomembranous enterocolitis

enterotoxin B

12

micrococci to bacitracin and lysozyme

sensitive

13

there is good growth of staph in...

thioglycollate rth

14

what are the methods for the coagulase test?

slide and tube coagulase method

15

tests for identifying facultative gram (+) cocci

catalase test
coagulase test

16

the selective media for staph are...

Mannitol salt agar (MSA)
Columbia Collistin-Nalidixic agar (CNA)
Phenylethyl Alcohol agar (PEA)

17

staph to bacitracin and lysozyme

resistant

18

mild inflammation of hair follicles and oil glands

folliculitis

19

the reaction in DNAse test reaction

clearing of toluidine blue

20

what is this important marker in coagulase test?

clumping factor (cell bound coagulase)

21

multiple abscesses & focal lesions in the pulmonary parenchyma

pneumonia

22

this specie under Staph is an opportunistic organism and can be recovered from almost any clin. specimen

Staphylococcus aureus

23

what is used in coagulase test?

rabbit plasma

24

this organism is yellow pigmented, coagulase negative, colonize @ skin

micrococci luteus

25

the colony shows yellow pigments and is nonhemolytic

S. saprophyticus

26

this was previously called Enterotoxin F

Toxic Shock Syndrome Toxin - 1

27

this enzyme is active in the coagulase test

staphylocoagulase

28

results of Staph in modified oxidase

negative

29

this cytolytix toxin lyses RBCs

alpha hemolysin

30

result of micrococci in modified oxidase

positive

31

this is a product of Phage 1 group

Toxic Shock Syndrome Toxin - 1

32

lethal to PM leukocytes

associated with Panton-Valentine luekocidin (PVL)

gamma hemolysin

33

more aggresive than S. aureus and associated with catheter-related bacteremia and endocarditis

S. lugdunensis

34

a positive result in coagulase test would show

agglutination in the rabbit or pig plasma

35

what is used in mannitol fermentation?

mannitol salt

36

develops when organism is present in a wound or other focus of infection and gains entrance into the blood

osteomyelitis

37

this is an important staph bcoz of nosocomial infections and bcoz of concern of increasing drug resistance

S. aureus

38

"staphle" means

bunches ofr grapes

39

what needs to be done if negative result in tube coagulase test?

overnight incubation at room temp

40

predominantly hospital acquired

s. epidermidis

41

infection observed among IV drug users

bacteremia

42

a negative result in slide method must be confirmed using

tube coagulase method

43

the habitat of staph?

skin and mucous membrane

44

result in growth and fermentation on RSA

yellow color

45

what is a manifestation of positive result in tibe coagulase test

clot formation

46

this coagulase method is used to identify s. aureus

slide method

47

what is the proper order of identifyinf staph?

hemolysis test > coagulase > mannitol fermentation

48

positive result in novobiocin test indicates

s. epidermidis

49

fastidious bacteria require

CO2, hemin, menadione

50

culture of this shows nonhemolytic, white to gray colonies

S. epidermidis

51

this staph is susceptible to novobiocin

s. epidermidis

52

staph's anaerobic acid production from glucose

fermentative

53

there are numerous Gram (+) cocci PMNs in...

purulent exudates
joint fluids
aspirated secretions

54

other name of hyaluronidase

Durans-Reynal factor

55

larger and surrounded by erythema, highly contagious

bullous impetigo

56

negative result in catalase test indicates

streptococci/enterococci

57

staph infection is determined by?

virulence of the strain, size of inoculum and patient's immune system

58

result of staph to lysostaphin

sensitive

59

larger more invasive lesions, may progress into deeper tissues

carbuncles

60

when absorbed through the vaginal mucosa, this permits systemic effects

Toxic Shock Syndrome Toxin - 1

61

this Staph is resistant to novobiocin

S. saprophyticus

62

this causes sloughing of epidermal layer of skin

Exfoliative/Epidermolytic toxin

63

less lethal cytolytic toxins

WBC sestruction; injury to most cells in culture

delta hemolysin

64

negative result in novobiocin indicates

s. saprophyticus

65

this cytolytic toxin targerts the sphyngomyelin of RBCs (Sphyngomyelin C)

also called hot-cold lysine

beta hemolysin

66

cann occur in children with trauma to the extremities or patients with a history of rheumatoid arthritis

septic arthritis

67

a positive result in catalase test indicates

staph spp

68

indication of a positive result in tube coagulase test

extracellular free coagulase

69

cutaneous infections by S. aureus are considered

suppurative

70

a toxin induced disease commonly drug induced and resolve thru steroid therapy

toxin epidermal necrolysis

71

gram positive, catalase producing spherical

staphylococci

72

inflammation of the heart

endocarditis

73

T or F. specimens should be taken from the site of infection after appropriate cleansing of the surrounding area to avoid contamination by the skin microbiota

True

74

medium used in DNAse test rxn

Nutrient Agar-based medium

75

this enzyme is identified by the cell wall

Protein a

76

large, raised and with superficial abscesses

boils/furuncles

77

special considerations in over ight incubation of tube coagulase negative test

checked every hour due to false negative result if clots are dissolved by staphylokinase/fibrinolysis

78

a possible cause of menstruating-associated TSS

tampons

79

yellow color in growth and fermentation on RSA indcates

acid fermentation

80

enterotoxin responsible for food poisoning

A, B and D

81

may be UTIs associated with pyelonephritis and cystitis

S. saprophyticus

82

enzyme that destroys hyaluronic acid

hyaluronidase

83

these resemble staph except that they are coagulase negative

micrococci

84

negative result in tube coagulase indicates

no free coagulase

85

micrococci's anaerobic acid production from glucose

oxidative

86

a type of enterotoxin that are resistant to gastric and digestive juices

A and D

87

produced by Phage II group

Exfoliative toxin/Epidermolytic toxin

88

these are heat stable exotoxins

enterotoxins

89

positive teat in coagulase test indicates

s. aureus

90

associated with infection by this staph are IV catheters, CSF shunts, other prosthetic devices, use of implants

S. epidermidis

91

culture of this shows yellow, tan, orange colony, beta hemolytic

s. aureus

92

the only specie for micrococci

micrococci luteus

93

how will you determine if a staph is beta-hemolytic

there is a clear zone around the colony

94

this property of coagulase causes bacterial cells to agglutinate in plasma

clumping factor

95

which Staph is not a facultative anaerobe

S. saccharolyticus