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Flashcards in Streptococci, Enterococci Deck (162):
0

facultative anaerobe or aerotolerant anaerobe

strep

1

flora of intestine

enterococci

4

mediate adherence to host epithelial cells

lipoteichoic acid and protein F

5

Lancefield B

S. agalactiae

6

type of metabolism of strep and entero

lactic acid fermentation

7

partial lysis, greenish coloration

alpha hemolysis

11

nonLancefield

S. pneumoniae

12

complete lysis, clear zone

beta hemolysis

13

no lysis, no change in agar

nonhemolytic or gamma

14

small area of intact rbc, wider zone of complete hemolysis

alpha prime hemolysis

15

green coloration in alpha hemolysis is due to

metHb

16

pus producing strep

s. pyogenes

17

resistance to novobiocin

enterococci

18

classification accdg to presence of C-carbohydrates on cell wall

Lancefield grouping

19

opportunistic alpha/nonhemolytic strep in the upper resp tract

s. viridans

20

how is C.carbohydrate extracted from the cell wall

thru dilute HCl
heated 10mins

21

what antibiotic inhibits grp A streps

bacitracin

22

strep susceptible to bacitracin

S. pyogenes

23

resistant to sulfamethoxazole & trimethoprim

S. pyogenes

24

resistant to bacitracin and SXT

S. agalactiae

25

virulence of Grp A strep

hemolysis
hyaluronidase
streptokinase
streptodornase
deoxyribonuclease (ABCD) antigen
erythrogenic txn

26

the special hemolysin for strep

streptolysin

27

types of streptolysin

streptolysin O and S

28

antigenic, oxygen labile and higly infective streptolysin

O

29

nonantigenic, oxygen stable and leukocyte-lysing streptolysin

S

30

enzymes by streps that dissolve clots

streptokinase
streptodornase

31

toxin causing scarlet fever

erythrogenic toxin

32

test for susceptibility to scarlet fever

Dick's test

33

diagnostic test for scarlet fever

Schultz-Charlton test

34

beta hemolytic strep

S. pyogenes
S. agalactiae
S. dysgalactiae
S. equisimilis

35

principle of Schultz-Charlton

anti-erythrogenic toxin is injected and rash will blanch then fade

36

allows streps to resist phagocytosis

M protein molecule

37

prevents phagocytosis by masking antigens and remaining unrecognized

hyaluronic acid capsule

38

aids in adherence of streps to mucosal cells

M protein molecule

39

characteristics of the strep colonies

pinpoint

40

Gram (+), Catalase (-) spherical cells

streptococcus

45

principle of Dick's test

injection of erhthrogenic toxin and swelling/redness in (+)

50

Lancefield D

S. bovis gropu
E. faecalis
E. faecium

66

Lancefield C

S. dysgalactiae
S. equisimilis

73

Lancefield A streps

S. pyogenes

74

growth of strepto requires high CO2

capnophilic

75

test for recent infection

Antistreptolysin O test

76

lyses fibrin clot

streptokinase

77

dissolved purulent or fibrinous secretions

streptodornase

78

spreading factor or invasiveness

hyaluronidase

79

highly mitogenic proteins

superantigens

SpeA
SpeB
SpeC

80

incubation period of bacterial pharyngitis and tonsilities

1-4days

81

signs of bacterial pharyngitis and tonsilitis

sore throat
malaise
fever
headache

82

other name for bacterial pharyngitis and tonsilitis

strep throat

83

clinical infections of strep include

strep throat
pyodermal infectious impetigo
erysipelas
cellulitis
strep pyogenes inf.
rheumatic fever
acute glomerulonephritis

84

strep pyogenes inf has affinity for

kidney

85

the capsule of S. agalactiae

sialic acid

86

infection of strep agalactiae in newborn leads to

neonatal sepsis

87

infections associated with E. fecalis

UTIs
wound infection

88

infections associated with S. bovis

UTIs
endocarditis
septicemia

89

diagnosis for strep throat

throat culture or direct antigen detection (DAD)

90

examples of pyodermal infections

impetigo
erysipelas
cellulitis

91

pyodermal infection for young children 2-5y.o.

impetigo

92

acute spreading skin lesion

erythematous with a plainly demarcated but irregular edge

for elderly patients

erysipelas

93

deeper invasion type of pyodermal infection

associated with gangrene

cellulitis

94

symptom of scarlet fever

strawberry tongue (yellow-white with red papillae)

95

flesh eating disease

necrotizing fascitis

96

rapidly progressing inflammation and necrosis of the skin

necrotizing fascitis

97

entire organ system shut down leading to death

Streptococcal toxic shock syndrome

98

treatment for post-streptococcal sequelae

penicillin or erythromycin

99

examples of post-streptococcal sequelae

rhuematic fever
acute glumerulonephritis

100

where is sample for diagnosis swabbed?

posterior pharynx

101

specimen diagnosis for streps are observed after ____ for the presence of beta hemolytic step

24hrs

102

key tests for strep

Bacitracin susceptibility or PYR hydrolysis

103

composition if terminal position of agalactiae

sialic acid

104

loss of capsular sialic acid =

loss of virulence

105

infection of agalactiae to cattle

mastitis

106

presence of grp B strep in mother's vagina may lead to

neonatal sepsis

107

treatment for agalactiae infections

ampicillin + aminoglycosides

108

vertical transmission from the mother

early onset neonatal sepsis

109

agar for growing pyogenes and agalactiae

SBA

110

grayish white mucoid colonies with small zone beta hemolytic

S. agalactiae

111

smooth and transparent colony, well defined beta hemolytic

S. pyogenes

112

useful tests for agalactiae

hippurate test or CAMP test

113

positive for agalactiae in strep B carrot broth manifest

orange or red pigment

114

a negative in catalase test indicates

strep and entero

115

susceptible to bacitracin indicates

grp A

116

resistance to bacitracin indicates

need of CAMP test

117

CAMP test positive means

grp B

118

CAMP test negative indicates

possibly grp D or beta hemolytic strep not A B or D

119

strep with large colony forming beta hemolytic

S. dysgalactiae
S. equisimilis

120

strep that is an animal pathogen

S. equi sibsp. zooepidermicus

121

also known as pneumococcus

s. pneumoniae

122

containes antigen similar to C-carbohydrate

s. pneumoniae

123

capsule of s. pneumonaie

capsular polysaccharide

124

opsonization of capsule =

avirulence

125

infections with s. pneumoniae

sinusitis
otitis media
pneumonia
bacteremia
meningitis

126

caused by s. pneumoniae, anesthesia. alcoholism, malnutrition and viral infection

lobar pneumonia

127

pneumoniae vaccine for children

PCV7

128

pneumoniae vaccine for adults

PS23

129

colony with coin appreance

pneumoniae old culture

130

differentiating pneumoniae feom viridans

optochin susceptibility
bile susceptibility

131

normal microbiota of the upper resp tract, female genital tract and GIT

s. viridans

132

viridan means

green

133

5 groups under viridans strep

S. mitis
S. mutans
S. salivarius
S. bovis
S anginosus

134

these would not grow in nutrient broth w/ 6.5% NaCl

nonenterococci

135

infection by S. mutans

gingivitis and dental caries

136

bacterimic patients with tendecies to form abscesses

S. anginosus

137

bacterial edocarditis in native valves

S. mitis

138

virulence factor of S. viridans

polysacc capsule
cytolysin
extracellular dextran
cell-surface proteins

139

PYR (-)
LAP (+)

S. viridans

140

colonies are small, surrounded by a zone ilof alpha hemolysis

S. viridans

141

S viridans colonies are

small, alpha hemolytic

142

S. pyogenes colonies

are small, transparent and smooth

143

S. anginosus grown in pure culture has

honeysuckle or butterscotch odor

144

when there is no hemolysis in blood agar, do

bile esculin test

145

(-) in bile esculin test indicates

nonhemolytic strep

146

(+) in bioe esculin indicates

need to test with 6.5%NaCl

147

(+) in 6.5%NaCl indicates

enterococcus spp

148

(-) in 6.5% NaCl indicates

grp D strep

149

alpha hemolysis on sheep blood ahar means

optochin disk test

150

susceptibility to optochin susceptibility indicates

s. pneumoniae

151

resistance to optochin indicates

bile esculin test

152

negative in bil esculin for alpha hemolytic ondicates

viridans strep

153

natural inhabitants of intestinal tract

enterococci

154

enterococci sometimes produce ____

pseudocatalase

155

pseudocatalase would show

weak bubbling in catalase test

156

virulence of enterococci

ogrows under extreme consitions
cytolysin
antimircrobial resistance

157

culture for entero

trypticase soy or brain heart infusion agar supplemented with 5% sheep blood

158

easily identifie by ability to grow in the presence of tellurite

E. faecalis

159

Entero has ability to

produce acid in carbohydrate broth
hydrolyze arginine
tolerate 0.04% tellurite
utilize pyruvate
motility
growth around 100 microgram efrotomycin acid disk

160

abiotrophia and granulicatella are

PYR (+)
bile esculin (-)
gram (+)

161

cure for abiotrophia and granulicatella

surgery

162

thiol and pyridoxal

abiotrophia
granulicatella

163

common airborne organism

aerococcus

164

occur as tetrads or clusters in broth

aerococcus

165

aerococcus in colonies shows

tetrads or clusters

166

aerococcus na - both pyr and bile

A. urinae

167

aerococcus na + pyr and bile

A. viridans

168

onlyalpha hemolytic strep ba susceptible to optochin

s. pneumoniae

169

easily decolorized on grams stain

Gemella

170

similar in morphology and habitat to viridans

Gemella

171

gram (+) cocci
previoualy called grp N strep

Lactococcus

172

catalase (-) gram (+) resistant to vancomycin

leuconostoc

173

found mainly in dairy products

lactic acid

174

resistant to vancomycin, bile esculin (+)

Pediococcus

175

flora of intestine, resistant to vancomycin

enterococci

176

Lancefield classification scheme is based on

extraction of C carbohydrate

177

use of bacitracin susceptibility test

identify grp A strep in throat culture

178

CAMP stands for

Christi, Atkins, Munch-Petersen

179

use of CAMP test

identify grp B strep

180

procedure in CAMP tests

streaked perpendicularly to a beta hemolytic staph aureus

181

end result of (+) hippurate hydrolysis

purple color

182

what us the purple color in hippurate hydrolysis test

ninhydrin reacting with alpha amino acid

183

use of hippurate hydrolysis test

grp B vs beta hemolytic

184

PYR stands for

pyrrolidonyl-alpha-naphthylamide

185

use of PYR hydrolysis

Beta hemolytic grp A and nonhemo grp D

186

more specific to pyogens than bacitracin

PYR hydrolysis

187

LAP stands for

Leucin aminopeptidase

188

principle of LAP

hydrolyzes peptide bonds adjacent to free amino acids

189

end color of LAP test

red color

190

red color in LAP is produced thru addition of

para-dimethylaminocinnamaldehyde rgt (DMACA)

191

use of Voges- Proskauer test

distinguish small colony-forming beta hemolytic anginosus grp from large colony forming pyogenic strain of grp A and C antigens

192

(+) result in Voges-Proskauer shows

red or pink color

193

principle of Voges-Proskauer

detects acetoin production from glucose

194

principle of BGUR test

detects action of BGUR

195

BGUR stands for

beta D glucoronidase