Synthetic Biology Flashcards

1
Q

Top-down synthetic biology involves…

A

modifying the genetic makeup of an organism in order to produce desirable products

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2
Q

Bottom-up synthetic biology involves…

A

Synthetic molecule systems produced in order to carry our biological processes

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3
Q

Biobrick parts are DNA sequences containing _ and _ regions for insertion

A

functional sequences

enzyme-digestible

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4
Q

Devices are _ sets that perform _-cellular function

A

biobrick

sub-cellular

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5
Q

Systems are sets of _

A

devices

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6
Q

Synthetic biology has an emphasis on _ and _ design, it has a heavy reliance on _

A

standardization
rational design
synthetic DNA technology

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7
Q

Synthia strategy is to…

A
  1. Synthesize systems and combine into a synthetic genome in E.coli
  2. Propagate as a centromeric plasmid in yeast
  3. Transplanted into mycoplasma
  4. Tested for gene essentiality, redesign and cycle
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8
Q

CRISPR-Cas9 is a tool for _ by employing _ in order to selectively target specific sites

A

genome editing

gRNAs

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