Techniques to investigate unknown compound

Question 1

How to separate compounds from a mixture

Answer 1

Thin Layer Chromatography

Question 2

How to get the chemical formula of an unknown compound

Answer 2

Mass spectrometry, separates by charge/weight ratio

Question 3

How to get chemical structure of unknown compound

Answer 3

NMR

Question 4

How to determine mechanism of action of known compound

Answer 4

Cell culture assay