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Flashcards in Test 1 Deck (40):
1

Where do contaminated items go?

In the biohazard bag - with the exception of glass or sharp objects

2

where do used test tubes go

in designated test tube rack to be autoclaved

3

where do used pipettes go

into provided container

4

where do used slides and covers go

in the glass box

5

media

nutrient solution used to cultivate or grow bacteria

includes broth and agar

6

growth characteristics in broth include

sediment
pellicle
turbid

7

sediment

growth at the bottom of the broth

8

pellicle

growth at the top of the broth

9

turbid

growth throughout the broth

10

growth characteristics of petri dish include

colony form, margin, and elevation

11

colony form can be

circular
irregular

12

colony margin can be

lobate
entire

13

colony elevation can be

flat
raised
convex

14

irregular colonies are typically

lobate
either raise or flat

15

circular colonies are typically

entire
either flat, raise, or convex

16

broth to broth transfer

o Sterilize loop for 10 seconds. Allow to cool.
o Sterilize the mouth of the test tube
o Insert sterile loop into tube and pick up loopful of culture
o Remove loop and sterilize mouth of tube again
o Replace tube cap
o Pick up tube to inoculate and sterilize the mouth
o Slide the loop into the tube. No need to rattle
o Remove the loop and sterilize the mouth again
o Replace the cap and return tube to rack
o Sterilize the loop

17

plate to slant transfer

o Select and isolated colony on plate
o Sterilize the loop
o Lift petri plate lid just enough to pick up the colony on the loop. Replace the lid.
o Pick up agar slant and remove lid. Sterilize the mouth.
o Inoculate the slant by gently moving the loop back and forth over the agar surface starting at the bottom and moving to the top.
o Sterilize the mouth of the tube
o Replace the cap
o Sterilize the loop

18

taxis

ability to move in a specific direction

19

positive taxis

move towards an advantageous stimuli

20

negative taxis

move away a harmful stimuli

21

monotrichous

1 flagella

22

amphitrichous

1 flagella at each end

23

pertrichous

multiple flagellus

24

1 flagella

monotrichous

25

1 flagella at each end

amphitrichous

26

multiple flagella

pertrichous

27

how does an autoclave kill cells

pressurized, moist, high heat

28

different between an isolated colony and a pure culture

isolated colony is found on a plate

pure culture may be on a plate or in a broth

29

<30 colonies on a pour plate

too few to count

30

30-300 colonies on a pour plate

count individual colonies

31

>30 colonies on a pour plate

too numerous to count

32

What do you use when there are too many colonies to count?

Quebec colony counter

33

How to estimate colonies

- place on Quebec colony counter
- pick 5 squares and count the colonies in each
- calculate the average number of colonies in the 5 squares
- multiply this average by 78

34

When using a Quebec colony counter, do not use...

squares that are not whole squares, ie are cut off

35

what are you trying to compute with the concentration of viable cells - CFU/ml

the number of cells present in the original sample

36

What do you need to calculate CFU/ml

- number of colonies on your plate
- dilution factor used on the plate

37

When is CFU/ml used?

to calculate contamination of food

38

What is the procedure used to gradually decrease the concentration of a sample in sterile water before making pour plate?

serial dilution

39

The plate had a sample diluted to 10^-4. There are 60 colonies. What is the cells/ml?

600,000

40

In a 1 ml pipette, where do you fill the liquid to transfer 1 ml of sample?

to the 0