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Flashcards in Tissues Deck (26):
1

4 types of tissues:

Epithelium
Connective
Muscular
Nervous

2

Anatomy -

branch of science that deals with external form and internal organization of both plants and animals

3

Anatomy can be subdivided into several component parts based on

by which structures are revealed (using a scalpel to see structures) and the size of the subunit studied (microscopic)

4

Histology encompasses

gross anatomy and microscopic anatomy

5

Gross anatomy

structures visible to the naked eye

6

Microscopic anatomy -

deals with structures beyond the reach of the naked eye

7

Microscopic anatomy contains what studies?

• Organology - study of organs
• Histology - study of tissues
• Cytology - study of cells

8

Two important considerations with regards to mythology

1) the kind of microscope to be used
2) preparation of tissue or organ in a manner suitable for viewing with the microscope

9

Microscopes are classified:

1) light source used
2) degree of resolution

10

Types of visible light microscopes:

• optical
• Polarizing
• Phase contrast
• Interference
• Dark field
• Ultraviolet
• X-ray
• Transmission electron (TEM)
• Scanning electron (SEM)
• compound

11

THE PRINCIPLE INSTRUMENT IN HISTOLOGY AND CYTOLOGY IS COMPOUND

Compound microscope

12

2 additional techniques other than microscopes:

• auto radiology of chemical localization
• Immunocytochemistry - method which enables investigator to localize sites of antibody-antigen rxns

13

Stains uses:

• Nuclear rgn - hematoxylin (basic stain)
• Cytoplasmic rgn - eosin (acidic stain)

14

How to prepare dead tissue:

1) Obtain tissue
- Biopsy
- Surgical excision
- Postmortem dissection
2) Fixation - stop all biological activity
3) Dehydration - inc alcohol %
4) Clearing - use soln/chemical thats I’m able (xylol)
5) Embedding/Infiltration - use hot wax to stick it to
6) Sectioning
7) staining and mounting

15

Cell Theory:

• all living matter is composed of cells
• Cells arise from preexisting cells
• All the metabolic rxns of the living organism take place w/in the cell

16

The body is composed of 3 elements:

1) Cells
2) intercellular substances
3) body fluids (blood and lymph)

17

Cytoplasm

Metabolic and synthetic activity

18

Nucleus

Differentiation and function

19

Protoplasm

The living substance of all cells

20

Two different forms of protoplasm

Cytoplasm and nucleoplasm

21

What substances are the protoplasm comprised of

Water - 75%
Protein - 10-20%
Lipids 2-3%
Carbohydrates - 1%
Salt - 1%

22

Characteristics of a protoplasm

Irritability
Conductivity
Contractility & Motility
Metabolism
Growth
Reproduction
Absorption
Secretion
Excretion
Respiration

23

What chemical processes are a part of metabolism

Anabolism and catabolism

24

What is part of absorption?

Pinocytosis (cell drinking)
Phagocytosis (cell eating)

25

Cytoplasmic regions include

Organelles and inclusions

26

Which is the correct cell model?

The fluid mosaic model