Transcription

Question 1

what does transcription do?

Answer 1

DNA becomes RNA (3 types)

Question 2

where?

Answer 2

nucleus

Question 3

4 key points

Answer 3

• anti parallel
• complementary
• mRNA from 5 to 3
• U instead of T

Question 4

Types of RNA

Answer 4

• mRNA (protein synthesis)
• rRNA (ribosomes)
• tRNA (transfer AA from cytoplasm to ribosome)

Question 5

Initiation

Answer 5

• RNA poly finds the two promoter sequences
• one of them is TATA box. 2nd one is for extra insurance
• RNA attaches to template strand (3 to 5)

Question 6

Elongation

Answer 6

• RNA poly and other proteins make transcription complex which uwinds DNA
• nucleotides pair with template strand
• DNA reanneals

Question 7

Termination

Answer 7

• RNA poly meets terminator sequence which is 2 terminators in a row.
• mRNA separates from RNA poly which detaches from DNA

Question 8

Post transcription proccess?

Answer 8

• mRNA has a 5’ cap added on which will help start translation later
• poly A tail added which will help elongate life of mRNA. each time a protein is synthesized from mRNA, it shortens a bit
• spliceosomes/snRP’s remove introns