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Flashcards in Transcription Deck (16):
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Transcription in prok
Dna->

DNA -> mRNA
-takes place in the cytoplasm

1

Transcription in euk
DNA ->

DNA-> hnRNA
-takes place in the nucleus

2

What is a gene

DNA sequence that codes for a protein

3

What do we want to transcribe in transcription?

A gene

4

How many strands do we copy into RNA?

-only one strand
---the "template strand" or "anti-sense"
-in replication you copy both strands

5

What is the difference between the comlementary DNA strand and the new RNA strand?

-Uracil instead of thymine

6

Promoter

-attracts RNA pol to come and bind to the gene

7

Start site

Where tracription begins
-the beginning of the gene after the promoter

8

What pulls the strands apart

RNA pol

9

What do you do once you make the strand of RNA?

-depends if you are a prok or euk
-prok: coupled to translation because both take place in the cytoplasm

10

mRNA
tRNA
rRNa

-mRNA is the only one that codes for proteins
-tRNA and rRNA are involved in translation of the mRNA
-in prok all three are made by the same pol
-in euk there are three dif pol that make them separate

11

RNA POL I
RNA POL II
RNA POL III

I- makes rRNA
II- makes mRNA
III-makes trRNA

12

Promoters in prok vs euk

-prok: there is a "-35" element and a
"-10" element called the pribnow box rich in A and T

-euk: tata box at "-25" rich in A and T

13

Euk splicing

-Only in euk
-exons code for part of the protein
-introns need to be cut out and the exons are spliced together
-you attach a 5' cap at one end of splicing and a polyAtail
-it is then shipped out of the nucleus and makes it to the ribosomemfor translation

14

Where does transcription begin?

-begins at the start site
-binding begins at the promoter region

Replication starts at the origin of replication

15

Reverse transcriptase

Makes DNA from an RNA template
-reverse of transcription
-we get him from retro viruses