Transcription

Question 1

Bacterial RNA Pol

Answer 1

Produces m,r,tRNA

sigma subunit determines DNA binding specificity

Question 2

Bacterial transcription initiation

Answer 2

-10box and -35box dictates transcription start site

Sigma factor increases affinity for specific promoter sequences

Question 3

Sigma factors

Answer 3

sigma70 for housekeeping

sigma32 for heat shock genes

Question 4

Termination of bacterial transcription

Answer 4

Rho-dependent termination - GC-rich region causes formation of hairpin in mRNA
Rho-independent termination - Rho factor aids RNA pol dissociation by unwinding mRNA from DNA

Question 5

Euk RNA Polymerases

Answer 5

RNAPol I: rRNA
RNAPol II: mRNA
RNAPol III: tRNA

Question 6

Euk Initiation of Transcription

Answer 6

TATA boxes, CCAAT box and GC box

Question 7

Prokaryotic Gene Regulation

Answer 7

Sigma Factor
Repressors and Inducers - lac operon
Attenuation - Trp operon

Question 8

Eukaryotic Gene Regulation

Answer 8

Acetylation of histones (HATs open up chromatin and increase transcription and HDACs opposite)
Transcription Factors - eg. NFkB
Alternative Splicing
smallRNAs (RNAi)